Host: Rabbit
Target Protein: Caspase 3
Immunogen Range: 11-120/277
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 25402
Swiss Prot: P55213
Source: KLH conjugated synthetic peptide derived from rat Caspase 3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:500-2000)
ELISA(1:5000-10000)
Predicted Molecular Weight: 28
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lung lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-2593R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-2593R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-2593R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.