bs-2592R-HRP [Conjugated Primary Antibody]
JNK1+2+3 Polyclonal Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: JNK1+2+3

Immunogen Range: 151-250/384


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2.64E+14

Swiss Prot: Q61831

Source: KLH conjugated synthetic peptide derived from mouse JNK1/2/3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK1/JNK3. In turn, MAPK1/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 42-47


Cross Reactive Species: Human
Mouse
Rat
Pig
Chicken

Predicted Cross Reactive Species: Dog
Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang X et al. Deoxynivalenol induces toxicity and apoptosis in piglet hippocampal nerve cells via the MAPK signaling pathway.(2018) Toxicon.18:30386-6Read more>>
  • Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437.Read more>>
  • Fu S et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling.Int Immunopharmacol. 2019 Jun;71:1-6. Read more>>
  • Wen Y et al. NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway. Oxid Med Cell Longev. 2019 May 9;2019:4578175.Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Chi Q et al. Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen. Oxid Med Cell Longev. 2019 Jul 31;2019:8061823. Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124. Read more>>
  • Pan Li et al. Curcumin metabolites contribute to the effect of curcumin on ameliorating insulin sensitivity in high-glucose-induced insulin-resistant HepG2 cells. J Ethnopharmacol. 2020 Sep 15;259:113015.Read more>>
  • Yijie Zhang. et al. Effects of SP600125 and hypothermic machine perfusion on livers donated after cardiac death in a pig allograft transplantation model. Eur J Med Res. 2021 Dec;26(1):1-11Read more>>
  • Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873Read more>>
  • Wenbo Ge. et al. 17-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro. Mol Immunol. 2020 Nov;127:21Read more>>
  • Gerson G Contreras-Chavez. et al. Changes in Appetite Regulation-Related Signaling Pathways in the Brain of Mice Supplemented with Non-nutritive Sweeteners. 2020 Oct 31Read more>>
  • Le Zhuang. et al. Evaluation of the effects of IL?22 on the proliferation and differentiation of keratinocytes in?vitro. Mol Med Rep. 2020 Oct;22(4):2715-2722Read more>>
  • Fang Jia. et al. Cytotoxicity and anti-inflammatory effect of a novel diminazene aceturate derivative in bovine mammary epithelial cells. Res Vet Sci. 2021 Jul;137:102Read more>>
  • Qihe Tang. et al. Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-B Pathways Against Klebsiella pneumonia Infection. Front Pharmacol. 2021; 12: 651664Read more>>
  • Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brainCliver axis in rats. 2021 May 18Read more>>
  • Jianye Yang. et al. Astragalus polysaccharide attenuates LPS-related inflammatory osteolysis by suppressing osteoclastogenesis by reducing the MAPK signalling pathway. 2021 Jun 02Read more>>
  • Lili Liu. et al. Rutin Ameliorates Cadmium-Induced Necroptosis in the Chicken Liver via Inhibiting Oxidative Stress and MAPK/NF-B Pathway. 2021 Jun 06Read more>>
  • Junfeng Ke. et al. CTI-2 Inhibits Metastasis and Epithelial-Mesenchymal Transition of Breast Cancer Cells by Modulating MAPK Signaling Pathway. Int J Mol Sci. 2021 Jan;22(22):12229Read more>>
  • Zhang, Rongrong. et al. Compound traditional Chinese medicine dermatitis ointment ameliorates inflammatory responses and dysregulation of itch-related molecules in atopic dermatitis. Chin Med-Uk. 2022 Dec;17(1):1-19Read more>>
  • Xzaviar K.V. Solone. et al. MAP kinases differentially bind and phosphorylate NOS3 via two unique NOS3 sites. 2022 Feb 19Read more>>
  • Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-BHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453Read more>>
  • Wen, Yukang. et al. Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages. VET RES. 2022 Dec;53(1):1-15Read more>>
  • Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 AugRead more>>
  • Dan-Ping Xie. et al. Peroxiredoxin 5 protects HepG2 cells from ethyl -carboline-3-carboxylate-induced cell death via ROS-dependent MAPK signalling pathways. J CANCER. 2022 Sep 6;13(11):3258-3267Read more>>
  • Mingjuan Yang. et al. Rosmarinic acid potentiates and detoxifies tacrine in combination for Alzheimer's disease. PHYTOMEDICINE. 2022 Dec;:1546Read more>>
  • Zhang Xiangjun. et al. DAD3 targets ACE2 to inhibit the MAPK and NF-B signalling pathways and protect against LPS-induced inflammation in bovine mammary epithelial cells. VET RES. 2022 Dec;53(1):1-13Read more>>
  • Yiming Bi. et al. -Sitosterol Suppresses LPS-Induced Cytokine Production in Human Umbilical Vein Endothelial Cells via MAPKs and NF-<i></i>B Signaling Pathway. EVID-BASED COMPL ALT. 2023 Jan 03;2023:924109Read more>>
  • Yang Liu. et al. Isochlorogenic Acid C Restrains Erk/JNK/NF-B Signaling to Alleviate Inflammatory Response and Promote Cell Apoptosis. J FOOD BIOCHEM. 2023;2023:5547108Read more>>
  • Shu Zeng. et al. Inhibition of mGluR5 ameliorates lipid accumulation and inflammation in HepG2 cells. BIOCHEM BIOPH RES CO. 2023 Apr;653:1Read more>>
  • Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Hao Huang. et al. MAP4K4 is involved in the neuronal development of retinal photoreceptors. EXP EYE RES. 2023 Aug;233:109524Read more>>
  • Jing Zhang. et al. Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-B and restoring lipid production in Caco-2 cell line. J FUNCT FOODS. 2023 Aug;107:105709Read more>>
  • Zhong Shenjie. et al. Bmp8a deletion leads to obesity through regulation of lipid metabolism and adipocyte differentiation. COMMUN BIOL. 2023 Aug;6(1):1-13Read more>>
  • Lemiao Zhong. et al. Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis. VIRULENCE. 2023 Nov 16Read more>>
  • Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>
  • Keyi Nong. et al. Effect of the Pseudopleuronectes americanus-derived Pleurocidin on DSS-induced Ulcerative colitis in mice and its preliminary molecular mechanisms. INT IMMUNOPHARMACOL. 2024 Mar;130:111757Read more>>
  • Keyi Nong. et al. Potential effects and mechanism of flavonoids extract of Callicarpa nudiflora Hook on DSS-induced colitis in mice. PHYTOMEDICINE. 2024 Jun;128:155523Read more>>
  • Jialei Tian. et al. Chondroitin sulphate modified MoS2 nanoenzyme with multifunctional activities for treatment of Alzheimer's disease. INT J BIOL MACROMOL. 2024 May;266:131425Read more>>
VALIDATION IMAGES