bs-2590R [Primary Antibody]
NRSF/REST Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NRSF/REST

Specificity: KO-Validated

Immunogen Range: 401-450/1097


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5978

Swiss Prot: Q13127

Source: KLH conjugated synthetic peptide derived from human NRSF

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 121


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhu NW et al. Possible mechanisms of lycopene amelioration of learning and memory impairment in rats with vascular dementia. Neural Regen Res. 2020 Feb;15(2):332-341. Read more>>
  • uqing Yeet al. The chromatin remodeler SRCAP promotes self\renewal of intestinal stem cells. EMBO J. 2020 Jul 1;39(13):e103786.Read more>>
  • Crews Fulton T.. et al. HMGB1 neuroimmune signaling and REST-G9a gene repression contribute to ethanol-induced reversible suppression of the cholinergic neuron phenotype. MOL PSYCHIATR. 2023 Jul;:1-14Read more>>
  • Kojiro Ishibashi. et al. Astrocyte-induced mGluR1 activates human lung cancer brain metastasis via glutamate-dependent stabilization of EGFR. DEV CELL. 2024 Feb 02Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti NRSF/REST Polyclonal Antibody,Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded mouse brain labeled with Anti-NRSF/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Raji lysates probed with NRSF Polyclonal Antibody, Unconjugated (bs-2590R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: Hela KO NRSF cell lysates probed with NRSF Polyclonal Antibody, Unconjugated (bs-2590R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: human hela cell lysates; Lane 2: bs-2590R IP in human hela cell lysates; Lane 3: native rabbit IgG IP in human hela cell lysates probed with NRSF/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:150 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.