bs-2572R [Primary Antibody]
FPR1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FPR1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2357

Source: KLH conjugated synthetic peptide derived from human FPR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

High affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang et al. Formylpeptide Receptors Promote the Migration and Differentiation of Rat Neural Stem Cells. (2016) Sci.Re. 6:25946Read more>>
  • Franken et al. Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates. (2015) Sci.Rep. 5:12940Read more>>
  • Ana Paula Girol. et al. Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis. Cells-Basel. 2021 Nov;10(11):317Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-FPR1 Polyclonal Antibody, Unconjugated(bs-2572R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG,Alexa Fluor 647 conjugated(bs-0295G-AF647)used at 1:200 dilution for 40 minutes at 37°C.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-FPR1 Polyclonal Antibody, Unconjugated (bs-2572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Anti-FPR1 Polyclonal Antibody, Unconjugated(bs-2572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat kidney labeled with Anti-FPR1 Polyclonal Antibody, Unconjugated (bs-2572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Human U-87MG cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human U937 cell lysates; Lane 4: Human SW480 cell lysates probed with FPR1 Polyclonal Antibody, Unconjugated (bs-2572R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.