bs-2559R [Primary Antibody]
eIF4EBP1/4EBP1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: e4EBP1/4EBP1

Specificity: 60-70% sequence similarity to 4EBP2

Immunogen Range: 95-118/118


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1978

Source: KLH conjugated synthetic peptide derived from human 4E-BP1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen N et al. Carbohydrate response element\binding protein regulates lipid metabolism via mTOR complex1 in diabetic nephropathy. J Cell Physiol. 2021 Jan;236(1):625-640.Read more>>
  • Zhao Jiamin. et al. Sea buckthorn oil regulates primary myoblasts proliferation and differentiation in vitro. IN VITRO CELL DEV-AN. 2023 Dec;:1-12Read more>>
  • Yu Han. et al. High-precision bioactive scaffold with dECM and extracellular vesicles targeting 4E-BP inhibition for cartilage injury repair. MATER TODAY BIO. 2024 Aug;27:101114Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-eIF4EBP1/4EBP1 Polyclonal Antibody, Unconjugated (bs-2559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Lane 1: Human HepG2 cell lysates; Lane 2: Human HeLa cell lysates probed with eIF4EBP1 Polyclonal Antibody, Unconjugated (bs-2559R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.