bs-2540R
[Primary Antibody]
GLUT12 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GLUT12
Immunogen Range: 251-350/617
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 154091
Swiss Prot: Q8TD20
Source: KLH conjugated synthetic peptide derived from human GLUT12
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
GLUT12 belongs to a family of transporters that catalyze the uptake of sugars through facilitated diffusion. Thirteen different types of glucose/fructose transport carrier proteins designated as Glut 1-13 facilitate glucose/fructose transport across the cell membrane. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 68
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Chicken
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Pujol-Gimenez, Jonai, et al. "Expression of the Glucose Transporter GLUT12 in Alzheimers Disease Patients." Journal of Alzheimers Disease (2014).Read more>>
- Murakami, Ryuta, et al. "Immunoreactivity of glucose transporter 8 is localized in the epithelial cells of the choroid plexus and in ependymal cells." Histochemistry and Cell Biology (2016):1-6Read more>>
- Gil-Iturbe E et al. GLUT12 expression and regulation in murine small intestine and human Caco‐2 cells. (2018) J Cell Physiol. Oct 23. Read more>>
- Gil-Iturbe E et al. GLUT12 Expression in Brain of Mouse Models of Alzheimer's Disease. Mol Neurobiol. 2019 Aug 31.Read more>>
- Gil-Iturbe et al. Effect of aging and obesity on GLUT12 expression in small intestine, adipose tissue, muscle and kidney, and its regulation by docosahexaenoic acid and exercise in mice. Appl Physiol Nutr Metab. 2020 Mar 16.Read more>>
- Xiong Ying. et al. SLC2A12 of SLC2 Gene Family in Bird Provides Functional Compensation for the Loss of SLC2A4 Gene in Other Vertebrates. Mol Biol Evol. 2020 NovRead more>>
- Yoichi Chiba. et al. Glucose, Fructose, and Urate Transporters in the Choroid Plexus Epithelium. Int J Mol Sci. 2020 Jan;21(19):723Read more>>
- Pawe? Jan Stanirowski. et al. Placental expression of glucose transporters GLUT-1, GLUT-3, GLUT-8 and GLUT-12 in pregnancies complicated by gestational and type 1 diabetes mellitus.. 2021 Sep 23Read more>>
- Mingmei Li. et al. Hypoxia promotes the growth and metastasis of ovarian cancer cells by suppressing ferroptosis via upregulating SLC2A12. EXP CELL RES. 2023 Nov;:113851Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\n
This image has been kindly submitted by Efi Tsouko from the University of Houston.\n\nHuman LNCaP prostate cancer cells stained with Rabbit Anti-GLUT12 Polyclonal Antibody (bs-2540R)for 30 minutes at 4°C.Followed by secondary antibody AlexaFluor488 incubation for 30min 4°C in the dark.\n
Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, DAB staining.
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT12 ) polyclonal Antibody, Unconjugated (bs-2540R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
A-549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT12) polyclonal Antibody, Unconjugated (bs-2540R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric fundus adenocarcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with GLUT12 polyclonal antibody, unconjugated (bs-2540R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The MCF-7(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-GLUT12 antibody (bs-2540R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF647 (bs-40295G-BF647): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.