bs-2527R-FITC
[Conjugated Primary Antibody]
CD163/M130 Polyclonal Antibody, FITC Conjugated
DATASHEET
Host: Rabbit
Target Protein: CD163/M130
Immunogen Range: 1001-1121/1156
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9332
Swiss Prot: Q86VB7
Source: KLH conjugated synthetic peptide derived from human CD163/M130
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Conjugation: FITC
Excitation/ Emission: 494nm/518nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 130
Cross Reactive Species:
Human
Mouse
Rat
Dog
Pig
Others
Predicted Cross Reactive Species: Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Weng, Sherry, et al. "Vitamin D Deficiency Induces High Blood Pressure and Accelerates Atherosclerosis in Mice." PloS one 8.1 (2013): e54625.Read more>>
- Wang, Shan, et al. "5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts." Ultrasound in Medicine & Biology (2014).Read more>>
- Tepeköylü, Can, et al. "Alteration of inflammatory response by shock wave therapy leads to reduced calcification of decellularized aortic xenografts in mice." European Journal of Cardio-Thoracic Surgery (2014): ezu428.Read more>>
- Oh, Jisu, et al. "Deletion of Macrophage Vitamin D Receptor Promotes Insulin Resistance and Monocyte Cholesterol Transport to Accelerate Atherosclerosis in Mice." Cell Reports (2015).Read more>>
- Ge, L. P., et al. "Integration of nondegradable polystyrene and degradable gelatin in a core–sheath nanofibrous patch for pelvic reconstruction." International Journal of Nanomedicine 2015:10 3193–3201Read more>>
- Jain, Shardool, Thanh-Huyen Tran, and Mansoor Amiji. "Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis." Biomaterials (2015).Read more>>
- Franken, Lars, et al. "Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates." Scientific Reports5 (2015).Read more>>
- Jacobsen, Mette J., et al. "Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity." Journal of Diabetes Research (2015).Read more>>
- Wang, Lei, et al. "A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-κB and Caspase 3." International Journal of Nanomedicine 10 (2015): 6725.Read more>>
- Drel, Viktor R., et al. "Centrality of bone marrow in the severity of gadolinium-based contrast-induced systemic fibrosis." The FASEB Journal(2016): fj-201500188R.Read more>>
- Das, Subhamoy, et al. "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." Advanced Healthcare Materials (2016).Read more>>
- Dharmakumar, Rohan, and Ivan Cokic. "Assessment of iron deposition post myocardial infarction as a marker of myocardial hemorrhage." U.S. Patent Application No. 14/125,307.Read more>>
- Kugo, H. et al. "Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm." Sci. Rep. 6, 31268Read more>>
- Dinulovic, Ivana, et al. "PGC-1α modulates necrosis, inflammatory response, and fibrotic tissue formation in injured skeletal muscle." Skeletal Muscle 6.1 (2016): 38.Read more>>
- Bobi, Joaquim, et al. "Intracoronary Administration of Allogeneic Adipose Tissue–Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial Infarction." Journal of the American Heart Association 6.5 (2017): e005771.Read more>>
- Chen, Xionglin, et al. "Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation." Am J Transl Res 9.5 (2017): 2352-2362.Read more>>
- Yin, Li, Cuifang Chang, and Cunshuan Xu. "Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration." BioMed Research International 2017 (2017).Read more>>
- Gerzanich, Volodymyr, et al. "Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis." Journal of Neuroinflammation 14.1 (2017): 177.Read more>>
- Oh, Jisu, et al. "Deletion of JNK2 prevents vitamin-D-deficiency-induced hypertension and atherosclerosis in mice." The Journal of Steroid Biochemistry and Molecular Biology (2017).Read more>>
- Lee, Jin, et al. "Preventive Inhibition of Liver Tumorigenesis by Systemic Activation of Innate Immune Functions." Cell Reports 21.7 (2017): 1870-1882.Read more>>
- Dinulovic et al. PGC-1α modulates necrosis, inflammatory response, and fibrotic tissue formation in injured skeletal muscle. (2016) Skelet.Muscl. 6:38Read more>>
- Tauchi et al. Tumor-associated macrophages induce capillary morphogenesis of lymphatic endothelial cells derived from human gastric cancer. (2016) Cancer.Sc. 107:1101-9Read more>>
- Oh et al. Deletion of macrophage Vitamin D receptor promotes insulin resistance and monocyte cholesterol transport to accelerate atherosclerosis in mice. (2015) Cell.Re. 10:1872-86Read more>>
- Chen et al. Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing. (2015) Sci.Re. 5:18104Read more>>
- Riuzzi et al. Levels of S100B protein drive the reparative process in acute muscle injury and muscular dystrophy. (2017) Sci.Re. 7:12537Read more>>
- Fernandez-Bustamante et al. Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats. (2015) PLoS.On. 10:e0130764Read more>>
- Yin et al. Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration. (2017) Biomed.Res.Int. 2017:8428926Read more>>
- Kobori et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. (2018) Front.Immunol. 9:334Read more>>
- Kobori et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. (2018) Front.Immunol. 9:334Read more>>
- Protti et al. Bone marrow transplantation modulates tissue macrophage phenotype and enhances cardiac recovery after subsequent acute myocardial infarction. (2016) J.Mol.Cell.Cardiol. 90:120-8Read more>>
- Tran et al. Modulation of Macrophage Functional Polarity towards Anti-Inflammatory Phenotype with Plasmid DNA Delivery in CD44 Targeting Hyaluronic Acid Nanoparticles. (2015) Sci.Rep. 5:16632Read more>>
- Hamasaki et al. Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis. (2018) Sci.Rep. 8:5901Read more>>
- Tatebayashi K et al. Adipose-derived stem cell therapy inhibits the deterioration of cerebral infarction by altering macrophage kinetics. Brain Res. 2019 Feb 2. pii: S0006-8993(19)30061-7.Read more>>
- Gill N et al. The immunophenotype of decidual macrophages in acute atherosis. Am J Reprod Immunol. 2019 Feb 8:e13098.Read more>>
- "Liu GZ et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model.Naunyn Schmiedebergs Arch Pharmacol. 2019 Mar 9."Read more>>
- Zhou S et al. High Expression of Angiopoietin-like Protein 4 in Advanced Colorectal Cancer and its Association with Regulatory T Cells and M2 Macrophages. Pathol Oncol Res. 2019 Jul 1.Read more>>
- Moser PT et al. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue Eng Part A. 2019 Oct 30. Read more>>
- Akazawa Y et al. M-CSF Receptor Antagonists Inhibit the Initiation and Progression of Hepatocellular Carcinoma in Mice. Anticancer Res. 2019 Sep;39(9):4787-4794. Read more>>
- Shao N et al. A Multi‐Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy. Macromol Biosci. 2019 Nov 10:e1900255. Read more>>
- Thomas AJ et al. Macrophage CD163 expression in cerebrospinal fluid: association with subarachnoid hemorrhage outcome.J Neurosurg. 2018 Jul 1:1-7.Read more>>
- Thomas AJ et al. Macrophage CD163 expression in cerebrospinal fluid: association with subarachnoid hemorrhage outcome.J Neurosurg. 2018 Jul 1:1-7.Read more>>
- Tong?L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.Read more>>
- Philipp T Moser et al. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue Eng Part A. 2020 May;26(9-10):543-555.Read more>>
- Liang Wang et al. Clinical Significance of M1/M2 Macrophages and Related Cytokines in Patients with Spinal Tuberculosis. Dis Markers. 2020 May 20;2020:2509454.Read more>>
- Fan Guo #et al. Tumor-Associated CD163+ M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer. Cancer Manag Res. 2020 Jul 15;12:5831-5843.Read more>>
- Yang G et al. A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. Biomaterials. 2020 Oct;255:120155.Read more>>
- Lidija Gradi?nik. et al. Isolation, characterisation and phagocytic function of human macrophages from human peripheral blood. Mol Biol Rep. 2020 Sep;47(9):6929-694Read more>>
- Sha Liu. et al. Dual Cross-linked HHA Hydrogel Supplies and Regulates M2 for Synergistic Improvement of Immunocompromise and Impaired Angiogenesis to Enhance Diabetic Chronic Wound Healing. Biomacromolecules. 2020;21(9):3795C3806Read more>>
- Thiago DeSouza-Vieira. et al. Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance. Cell Rep. 2020 Oct;33:108317Read more>>
- Ayman J. Oweida. et al. Response to radiotherapy in pancreatic ductal adenocarcinoma is enhanced by inhibition of myeloid-derived suppressor cells using STAT3 anti-sense oligonucleotide. 2020 Oct 23Read more>>
VALIDATION IMAGES
U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163/M130 Polyclonal Antibody(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163/M130 Polyclonal Antibody,FITC Conjugated(bs-2527R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
The Hela(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): FITC conjugated Rabbit Anti-CD163 antibody (bs-2527R-FITC): 1 μg/10^6 cells; Isotype Control (orange): Rabbit-FITC IgG (bs-60295P-FITC). Blank control (black): PBS. Acquisition of 20,000 events was performed.