bs-2527R-Cy7 [Conjugated Primary Antibody]
CD163/M130 Polyclonal Antibody, Cy7 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD163/M130

Immunogen Range: 1001-1121/1156


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9332

Swiss Prot: Q86VB7

Source: KLH conjugated synthetic peptide derived from human CD163/M130

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Conjugation: Cy7

Excitation/ Emission: 743nm/767nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 130


Cross Reactive Species: Human
Mouse
Rat
Dog
Pig
Others

Predicted Cross Reactive Species: Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Weng, Sherry, et al. "Vitamin D Deficiency Induces High Blood Pressure and Accelerates Atherosclerosis in Mice." PloS one 8.1 (2013): e54625.Read more>>
  • Wang, Shan, et al. "5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts." Ultrasound in Medicine & Biology (2014).Read more>>
  • Tepeköylü, Can, et al. "Alteration of inflammatory response by shock wave therapy leads to reduced calcification of decellularized aortic xenografts in mice." European Journal of Cardio-Thoracic Surgery (2014): ezu428.Read more>>
  • Oh, Jisu, et al. "Deletion of Macrophage Vitamin D Receptor Promotes Insulin Resistance and Monocyte Cholesterol Transport to Accelerate Atherosclerosis in Mice." Cell Reports (2015).Read more>>
  • Ge, L. P., et al. "Integration of nondegradable polystyrene and degradable gelatin in a core–sheath nanofibrous patch for pelvic reconstruction." International Journal of Nanomedicine 2015:10 3193–3201Read more>>
  • Jain, Shardool, Thanh-Huyen Tran, and Mansoor Amiji. "Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis." Biomaterials (2015).Read more>>
  • Franken, Lars, et al. "Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates." Scientific Reports5 (2015).Read more>>
  • Jacobsen, Mette J., et al. "Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity." Journal of Diabetes Research (2015).Read more>>
  • Wang, Lei, et al. "A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-κB and Caspase 3." International Journal of Nanomedicine 10 (2015): 6725.Read more>>
  • Drel, Viktor R., et al. "Centrality of bone marrow in the severity of gadolinium-based contrast-induced systemic fibrosis." The FASEB Journal(2016): fj-201500188R.Read more>>
  • Das, Subhamoy, et al. "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." Advanced Healthcare Materials (2016).Read more>>
  • Dharmakumar, Rohan, and Ivan Cokic. "Assessment of iron deposition post myocardial infarction as a marker of myocardial hemorrhage." U.S. Patent Application No. 14/125,307.Read more>>
  • Kugo, H. et al. "Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm." Sci. Rep. 6, 31268Read more>>
  • Dinulovic, Ivana, et al. "PGC-1α modulates necrosis, inflammatory response, and fibrotic tissue formation in injured skeletal muscle." Skeletal Muscle 6.1 (2016): 38.Read more>>
  • Bobi, Joaquim, et al. "Intracoronary Administration of Allogeneic Adipose Tissue–Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial Infarction." Journal of the American Heart Association 6.5 (2017): e005771.Read more>>
  • Chen, Xionglin, et al. "Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation." Am J Transl Res 9.5 (2017): 2352-2362.Read more>>
  • Yin, Li, Cuifang Chang, and Cunshuan Xu. "Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration." BioMed Research International 2017 (2017).Read more>>
  • Gerzanich, Volodymyr, et al. "Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis." Journal of Neuroinflammation 14.1 (2017): 177.Read more>>
  • Oh, Jisu, et al. "Deletion of JNK2 prevents vitamin-D-deficiency-induced hypertension and atherosclerosis in mice." The Journal of Steroid Biochemistry and Molecular Biology (2017).Read more>>
  • Lee, Jin, et al. "Preventive Inhibition of Liver Tumorigenesis by Systemic Activation of Innate Immune Functions." Cell Reports 21.7 (2017): 1870-1882.Read more>>
  • Dinulovic et al. PGC-1α modulates necrosis, inflammatory response, and fibrotic tissue formation in injured skeletal muscle. (2016) Skelet.Muscl. 6:38Read more>>
  • Tauchi et al. Tumor-associated macrophages induce capillary morphogenesis of lymphatic endothelial cells derived from human gastric cancer. (2016) Cancer.Sc. 107:1101-9Read more>>
  • Oh et al. Deletion of macrophage Vitamin D receptor promotes insulin resistance and monocyte cholesterol transport to accelerate atherosclerosis in mice. (2015) Cell.Re. 10:1872-86Read more>>
  • Chen et al. Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing. (2015) Sci.Re. 5:18104Read more>>
  • Riuzzi et al. Levels of S100B protein drive the reparative process in acute muscle injury and muscular dystrophy. (2017) Sci.Re. 7:12537Read more>>
  • Fernandez-Bustamante et al. Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats. (2015) PLoS.On. 10:e0130764Read more>>
  • Yin et al. Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration. (2017) Biomed.Res.Int. 2017:8428926Read more>>
  • Kobori et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. (2018) Front.Immunol. 9:334Read more>>
  • Kobori et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. (2018) Front.Immunol. 9:334Read more>>
  • Protti et al. Bone marrow transplantation modulates tissue macrophage phenotype and enhances cardiac recovery after subsequent acute myocardial infarction. (2016) J.Mol.Cell.Cardiol. 90:120-8Read more>>
  • Tran et al. Modulation of Macrophage Functional Polarity towards Anti-Inflammatory Phenotype with Plasmid DNA Delivery in CD44 Targeting Hyaluronic Acid Nanoparticles. (2015) Sci.Rep. 5:16632Read more>>
  • Hamasaki et al. Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis. (2018) Sci.Rep. 8:5901Read more>>
  • Tatebayashi K et al. Adipose-derived stem cell therapy inhibits the deterioration of cerebral infarction by altering macrophage kinetics. Brain Res. 2019 Feb 2. pii: S0006-8993(19)30061-7.Read more>>
  • Gill N et al. The immunophenotype of decidual macrophages in acute atherosis. Am J Reprod Immunol. 2019 Feb 8:e13098.Read more>>
  • "Liu GZ et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model.Naunyn Schmiedebergs Arch Pharmacol. 2019 Mar 9."Read more>>
  • Zhou S et al. High Expression of Angiopoietin-like Protein 4 in Advanced Colorectal Cancer and its Association with Regulatory T Cells and M2 Macrophages. Pathol Oncol Res. 2019 Jul 1.Read more>>
  • Moser PT et al. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue Eng Part A. 2019 Oct 30. Read more>>
  • Akazawa Y et al. M-CSF Receptor Antagonists Inhibit the Initiation and Progression of Hepatocellular Carcinoma in Mice. Anticancer Res. 2019 Sep;39(9):4787-4794. Read more>>
  • Shao N et al. A Multi‐Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy. Macromol Biosci. 2019 Nov 10:e1900255. Read more>>
  • Thomas AJ et al. Macrophage CD163 expression in cerebrospinal fluid: association with subarachnoid hemorrhage outcome.J Neurosurg. 2018 Jul 1:1-7.Read more>>
  • Thomas AJ et al. Macrophage CD163 expression in cerebrospinal fluid: association with subarachnoid hemorrhage outcome.J Neurosurg. 2018 Jul 1:1-7.Read more>>
  • Tong?L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.Read more>>
  • Philipp T Moser et al. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue Eng Part A. 2020 May;26(9-10):543-555.Read more>>
  • Liang Wang et al. Clinical Significance of M1/M2 Macrophages and Related Cytokines in Patients with Spinal Tuberculosis. Dis Markers. 2020 May 20;2020:2509454.Read more>>
  • Fan Guo #et al. Tumor-Associated CD163+ M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer. Cancer Manag Res. 2020 Jul 15;12:5831-5843.Read more>>
  • Yang G et al. A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. Biomaterials. 2020 Oct;255:120155.Read more>>
  • Lidija Gradi?nik. et al. Isolation, characterisation and phagocytic function of human macrophages from human peripheral blood. Mol Biol Rep. 2020 Sep;47(9):6929-694Read more>>
  • Sha Liu. et al. Dual Cross-linked HHA Hydrogel Supplies and Regulates M2 for Synergistic Improvement of Immunocompromise and Impaired Angiogenesis to Enhance Diabetic Chronic Wound Healing. Biomacromolecules. 2020;21(9):3795C3806Read more>>
  • Thiago DeSouza-Vieira. et al. Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance. Cell Rep. 2020 Oct;33:108317Read more>>
  • Ayman J. Oweida. et al. Response to radiotherapy in pancreatic ductal adenocarcinoma is enhanced by inhibition of myeloid-derived suppressor cells using STAT3 anti-sense oligonucleotide. 2020 Oct 23Read more>>
VALIDATION IMAGES