Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated (bs-2527R) at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative. \n


Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated (bs-2527R) at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative.


L1 rat brain, L2 mouse uterus lysates probed (bs-2527R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 130kDa.


RSC96 cells probed with CD163/M130 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-2527R-A647) at 1:20 for 30 minutes compared to control cells (blue)and isotype control (orange).


THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163/M130 Polyclonal Antibody(bs-2527R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD163/M130 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated(bs-2527R-AF647)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Polyclonal Antibody, Unconjugated (bs-2527R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Polyclonal Antibody, Unconjugated (bs-2527R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarain Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Polyclonal Antibody, Unconjugated (bs-2527R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Polyclonal Antibody, Unconjugated (bs-2527R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Polyclonal Antibody, Unconjugated (bs-2527R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD163 Polyclonal Antibody, Unconjugated (bs-2527R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.