Host: Rabbit
Target Protein: CD30
Immunogen Range: 131-230/595
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 943
Swiss Prot: P28908
Source: KLH conjugated synthetic peptide derived from human CD30
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Conjugation: FITC
Excitation/ Emission: 494nm/518nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 62
Cross Reactive Species:
Human
Pig
Predicted Cross Reactive Species: Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- aijin Xia. et al. Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T?cell lymphoma treatment. MOL THER-ONCOLYTICS. 2023 Sep;30:86-102Read more>>
Raji probed with CD30 Polyclonal Antibody, FITC Conjugated (bs-2495R-FITC) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).
K562 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD30 Polyclonal Antibody(bs-2495R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raji cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD30 Antibody（bs-2495R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).