Host: Rabbit
Target Protein: Prostaglandin E Receptor EP4
Immunogen Range: 1-100/513
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 19219
Swiss Prot: P32240
Source: KLH conjugated synthetic peptide derived from mouse EP4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
ICC(1:100-500)
Predicted Molecular Weight: 53
Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Sample: Lane 1: Mouse Uterus tissue lysates Lane 2: Mouse Small intestine tissue lysates Lane 3: Mouse Kidney tissue lysates Lane 4: Mouse Prostate tissue lysates Primary: Anti-EP4 (bs-24918R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 55 kD
Ctll-2 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Prostaglandin E Receptor EP4 Antibody(bs-24918R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Uterus tissue lysates; Lane 2: Mouse Small intestine tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Prostate tissue lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-24918R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.