bs-2489R
[Primary Antibody]
CD9/MRP-1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD9/MRP-1
Immunogen Range: 101-200/228
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 928
Swiss Prot: P21926
Source: KLH conjugated synthetic peptide derived from human CD9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 25
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Pig
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jankovicova J et al.Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos.(2018) Int J Biol Macromol;123:931-938. Read more>>
- Jin J et al. Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte.Stem Cell Res Ther. 2019 Mar 15;10(1):95. Read more>>
- Chen Y et al. Epothilone B prevents lipopolysaccharide-induced inflammatory osteolysis through suppressing osteoclastogenesis via STAT3 signaling pathway. Aging (Albany NY). 2020 Jun 11;12(12):11698-11716.Read more>>
- Zhang J et al. Differential Expression of Urinary Exosomal Small RNAs in Idiopathic Membranous NephropathyBiomed Res Int.2020 Dec 29;2020:3170927.Read more>>
- Qinyu Ma. et al. Small extracellular vesicles deliver osteolytic effectors and mediate cancer\induced osteolysis in bone metastatic niche. J Extracell Vesicles. 2021 Feb;10(4):e12068Read more>>
- Mengmeng Liang. et al. Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1. Mol Ther-Nucl Acids. 2021 Mar;23:1191Read more>>
- Zhe Li. et al. SCAP knockout in SM22-Cre mice induces defective angiogenesis in the placental labyrinth. Biomed Pharmacother. 2021 Jan;133:111011Read more>>
- Junli Zhang. et al. Localized fluorescent imaging of multiple proteins on individual extracellular vesicles using rolling circle amplification for cancer diagnosis. J Extracell Vesicles. 2020 Nov;10(1):e12025Read more>>
- Xiaoyi Gao. et al. Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. Acs Sensors. 2021;6(10):3611C362Read more>>
- Zhao, Tiantian. et al. microRNA-15b-5p shuttled by mesenchymal stem cell-derived extracellular vesicles protects podocytes from diabetic nephropathy via downregulation of VEGF/PDK4 axis. 2021 Nov 22Read more>>
- Jana Antalkov. et al. Expression of _V integrin and its potential partners in bull reproductive tissues, germ cells and spermatozoa. INT J BIOL MACROMOL. Int J Biol Macromol. 2022 Jun;209:542Read more>>
- Fanchun Zeng. et al. Antagonizing exosomal miR-18a-5p derived from prostate cancer cells ameliorates metastasis-induced osteoblastic lesions by targeting Hist1h2bc and activating Wnt/_-catenin pathway. GENES DIS. 2022 JulRead more>>
- M.M. Yinhua Zhao. et al. Extracellular vesicles from hypoxia-pretreated adipose-derived stem cells regulate hypoxia/reoxygenation-induced human dermal microvascular endothelial apoptosis and autophagy in vitro. HELIYON. 2023 Feb; 9(2): e13315Read more>>
- Cui Jin. et al. Bone Mesenchymal Stem Cells Origin Exosomes are Effective Against Sepsis-Induced Acute Kidney Injury in Rat Model. INT J NANOMED. 2023 Dec 31Read more>>
- Yixuan Liang. et al. Exosomes-mediated transmission of standard bovine viral diarrhea strain OregonC24Va in bovine trophoblast cells. J REPROD IMMUNOL. 2024 Aug;164:104254Read more>>
- Zetao Wang. et al. Nano-vibration exciter: Hypoxia-inducible factor 1 signaling pathway-mediated extracellular vesicles as bioactive glass substitutes for bone regeneration. BIOACT MATER. 2024 Oct;40:46Read more>>
- Naihan Yuan. et al. CREG1 promotes bovine placental trophoblast cells exosome release by targeting IGF2R and participates in regulating organoid differentiation via exosomes transport. INT J BIOL MACROMOL. 2024 Aug;274:133298Read more>>
- Yuan Liu. et al. Mesenchymal stromal/stem cell tissue source and in vitro expansion impact extracellular vesicle protein and miRNA compositions as well as angiogenic and immunomodulatory capacities. J EXTRACELL VESICLES. 2024 Aug;13(8):e12472Read more>>
VALIDATION IMAGES
RSC96 cells probed with\tCD9/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R ) at 0.2ug for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse splenocytes probed with\tCD9/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD9 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD9 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Testis lysates; Lane 2: Mouse Placenta lysates; Lane 3: Rat Testis lysates probed with CD9/MRP-1 Polyclonal Antibody, Unconjugated (bs-2489R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.