bs-2485R [Primary Antibody]
JAK2(Tyr1007+Tyr1008) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: JAK2 Tyr1007+Tyr1008

Modification Site: Tyr1007+Tyr1008

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3717

Swiss Prot: O60674

Source: KLH conjugated synthetic phosphopeptide derived from human JAK2 around the phosphorylation site of Tyr1007/1008

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

JAK2 (Janus Activating Kinase 2) is a tyrosine kinase of the non-receptor type, that associates with the intracellular domains of cytokine receptors; JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin; it has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 131


Cross Reactive Species: Human
Mouse
Rat
Rabbit
Zebrafish

Predicted Cross Reactive Species: Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Iriyama, Noriyoshi, Yoshihiro Hatta, and Masami Takei. "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." Cancer Medicine (2016).Read more>>
  • Iriyama et al. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. (2016) Cancer.Med. 5:3223-3234Read more>>
  • Xu et al. Danshen attenuates cartilage injuries in osteoarthritis in vivo and in vitro by activating JAK2/STAT3 and AKT pathways. (2018) Exp.Anim. 67:127-137Read more>>
  • Li ZH et al. You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. J Ethnopharmacol. 2020 Jan 10;246:112240.Read more>>
  • Lei Yuet al. TMF, a natural dihydroflavonoid isolated from Scutellaria javanica Jungh, stimulates anticancer activity of s180 cancer-bearing mice, induces apoptosis, inhibits invasion and migration on HepG-2?cells. J Ethnopharmacol. 2020 Dec 5;263:113072.Read more>>
  • Chuan Liu. et al. Tibetan medicine Ershiwuwei Lvxue Pill attenuates collagen-induced arthritis via inhibition of JAK2/STAT3 signaling pathway. J Ethnopharmacol. 2021 Apr;270:11382Read more>>
  • Han Chen. et al. ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-B signaling pathway. Bmc Cancer. 2020 Dec;20(1):1-1Read more>>
  • Jiajia Wang. et al. TAK-242 ameliorates DSS-induced colitis by regulating the gut microbiota and the JAK2/STAT3 signaling pathway. Microb Cell Fact. 2020 Dec;19(1):1-17Read more>>
  • Yongxiang Li. et al. Diet containing stearic acid increases food reward-related behaviors in mice compared with oleic acid. Brain Res Bull. 2020 Nov;164:45Read more>>
  • Gerson G Contreras-Chavez. et al. Changes in Appetite Regulation-Related Signaling Pathways in the Brain of Mice Supplemented with Non-nutritive Sweeteners. 2020 Oct 31Read more>>
  • Xueli Bai. et al. Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma. Int Immunopharmacol. 2021 Oct;99:107996Read more>>
  • Yan Zhang. et al. Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimers Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis. J Agr Food Chem. 2021;69(47):14204C14214Read more>>
  • Zhaxi Mima. et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. INT IMMUNOPHARMACOL. 2022 Dec;113:10934Read more>>
  • Shen, Yue. et al. Hederagenin Suppresses Inflammation and Cartilage Degradation to Ameliorate the Progression of Osteoarthritis: An In vivo and In vitro Study. INFLAMMATION. 2022 Nov;:1-24Read more>>
  • Penglin Li. et al. Microbiota-derived 3-phenylpropionic acid promotes myotube hypertrophy by Foxo3/NAD+ signaling pathway. CELL BIOSCI. 2024; 14: 62Read more>>
  • Wang Jianan. et al. Association of attenuated leptin signaling pathways with impaired cardiac function under prolonged high-altitude hypoxia. SCI REP-UK. 2024 May;14(1):1-18Read more>>
  • Xin Zeng. et al. -linolenic acid ameliorates pentylenetetrazol-induced neuron apoptosis and neurological impairment in mice with seizures via down-regulating JAK2/STAT3 pathway. BRIT J NUTR. 2024 May;:1-34Read more>>
  • Zhao JinFeng. et al. Synthesis, biological activities and mechanistic studies of C20-ketone pachysandra alkaloids as anti-hepatocellular carcinoma agents. MOL DIVERS. 2024 Aug;:1-21Read more>>
VALIDATION IMAGES

HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK2(Tyr1007+Tyr1008) Polyclonal Antibody(bs-2485R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).