Host: Rabbit
Target Protein: IL4R
Immunogen Range: 201-300/825
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3566
Swiss Prot: P24394
Source: KLH conjugated synthetic peptide derived from human IL4R
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 88
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Danelli et al. Mast cells boost myeloid-derived suppressor cell activity and contribute to the development of tumor-favoring microenvironment. (2015) Cancer.Immunol.Res. 3:85-95Read more>>
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Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti IL-4R/CD124 Plastin Polyclonal Antibody, Unconjugated (bs-2458R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-IL-4R/CD124 Polyclonal Antibody, Unconjugated (bs-2458R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution
Human Raji Cells were fixed with 2% PFA (10min at room temperature). The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Staining with IL4R Polyclonal Antibody, Unconjugated (bs-2458R) was performed at 1:100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-PE, was used at a 1:200 dilution for 40 min at room temperature. Primary antibody staining (green) is compared to compared to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with IL-4R polyclonal antibody, unconjugated (bs-2458R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with IL-4R polyclonal antibody, unconjugated (bs-2458R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.