bs-2454R [Primary Antibody]
CCL19/MIP-3 beta Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CCL19/MIP-3 beta

Immunogen Range: 26-108/108


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24047

Swiss Prot: O70460

Source: KLH conjugated synthetic peptide derived from mouse CCL19

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 9.2


Predicted Cross Reactive Species: Human
Mouse
Rat
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Borrelli, G. M., et al. "Immunohistochemical Investigation of Metastasis-Related Chemokines in Deep-Infiltrating Endometriosis and Compromised Pelvic Sentinel Lymph Nodes." Reproductive Sciences (2015): 1933719115592711.Read more>>
  • Borrelli, Giuliano. Investigation of invasion factors in deep-infiltrating endometriosis. Diss. Freie Universität Berlin, 2015.Read more>>
  • Xinli Niu. et al. Naringenin attenuates experimental autoimmune encephalomyelitis by protecting the intact of blood-brain barrier and controlling inflammatory cell migration. J Nutr Biochem. 2021 Mar;89:10856Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-CCL19/MIP-3 beta Polyclonal Antibody, Unconjugated (bs-2454R) followed by conjugation to the secondary antibody


Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti CCL19/MIP-3 beta Polyclonal Antibody, Unconjugated (bs-2454R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 11kD. Observed band size: 11kD.