bs-2442R
[Primary Antibody]
MyoD1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MyoD1
Immunogen Range: 51-150/318
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 17927
Swiss Prot: P10085
Source: KLH conjugated synthetic peptide derived from mouse MyoD1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 35
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Dai, Yang, et al. "microRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1." Molecular and Cellular Biochemistry (2016): 1-10.Read more>>
- Shiratsuchi, Takaki, et al. "Sonic Hedgehog Supplementation Rapidly induces Myogenesis in Human Induced Pluripotent Stem Cells." Journal of St. Marianna University 6.2 (2015): 225-233.Read more>>
- Sente, Tahnee, et al. "Primary skeletal muscle myoblasts from chronic heart failure patients exhibit loss of anti-inflammatory and proliferative activity."BMC Cardiovascular Disorders 16.1 (2016): 1.Read more>>
- Kou YT et al. A transient protective effect of low-level laser irradiation against disuse-induced atrophy of rats. Lasers Med Sci. 2019 Apr 4.Read more>>
- Dawson LE et al. Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines. Sarcoma. 2020 Mar 26;2020:8647981.Read more>>
- Dawson LE et al. Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines. Sarcoma. 2020 Mar 26;2020:8647981.Read more>>
- Weng S et al. Improvement of muscular atrophy by AAVCSaCas9-mediated myostatin gene editing in aged mice. Cancer Gene Ther. 2020 Dec;27(12):960-975.Read more>>
- Jie Li. et al. MicroRNA-127-3p regulates myoblast proliferation by targeting Sept7. Biotechnol Lett. 2020 Sep;42(9):1633-1644Read more>>
- Siqi Liu. et al. Effects of lycopene on skeletal muscle-fiber type and high-fat diet-induced oxidative stress. J Nutr Biochem. 2021 Jan;87:108523Read more>>
- Xianglun Zhang. et al. Protective effect of resveratrol against hydrogen peroxide-induced oxidative stress in bovine skeletal muscle cells. Meat Sci. 2022 Mar;185:108724Read more>>
- Koike, Tatiana E.. et al. Glutamine supplementation improves contractile function of regenerating soleus muscles from rats. J Muscle Res Cell M. 2022 Feb;:1-11Read more>>
- Jing Zhang. et al. LncRNA SMARCD3-OT1 Promotes Muscle Hypertrophy and Fast-Twitch Fiber Transformation via Enhancing SMARCD3X4 Expression. INT J MOL SCI. 2022 Jan;23(9):451Read more>>
- Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212Read more>>
- Jin, Zhengyun. et al. Esophageal striated muscle hypertrophy and muscle fiber type transformation in MSTN knockout pigs. TRANSGENIC RES. 2022 May;:1-9Read more>>
- Sanghun Park. et al. Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat. BIOMOLECULES. 2022 Jun;12(6):838Read more>>
- Sheng-Zhong Han. et al. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation. J AGR FOOD CHEM. 2022;70(33):1012110133Read more>>
- Pengfei Shi. et al. Analysis of Promoter Methylation of the Bovine FOXO1 Gene and Its Effect on Proliferation and Differentiation of Myoblasts. ANIMALS. 2023 Jan;13(2):319Read more>>
- Jindi Zhao. et al. Mutation c.?379 C>T in DGAT1 affects intramyocellular lipid content by altering MYOD1 binding affinity. ANIM GENET. 2023 MaRead more>>
- Qingyu Ding. et al. N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway. LIFE SCI. 2023 Sep;329:121975Read more>>
- Genghua Chen. et al. Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation. CELL PROLIFERAT. 2023 Sep;:e13545Read more>>
- Lixia Sun. et al. Delta-like noncanonical notch ligand 2 regulates the proliferation and differentiation of sheep myoblasts through the Wnt/-catenin signaling pathway. J CELL PHYSIOL. 2024 Jul;:e31385Read more>>
VALIDATION IMAGES
Lane 1: mouse muscle lysates; Lane 2: mouse heart lysates probed with MyoD1 Polyclonal Antibody, Unconjugated (bs-2442R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded rat embryonic rhabdomyoma labeled with Anti-MyoD1/Myf3 Polyclonal Antibody, Unconjugated (bs-2442R) at 1:200 followed by conjugation to the secondary antibody
Lane 1: Rat muscle lysates; Lane 2: Mouse muscle lysates; Lane probed with MyoD1 Polyclonal Antibody, unconjugated (bs-2442R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Skeletal muscle Lysates; Lane 2: Mouse Tongue Lysates; Lane 3: Rat Skeletal muscle Lysates; Lane 4: Rat Tongue Lysates. Probed with MyoD1/Myf3 polyclonal Antibody, unconjugated (bs-2442R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.