bs-24331R [Primary Antibody]
RNF43 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RNF43

Immunogen Range: 401-500/783


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 54894

Swiss Prot: Q68DV7

Source: KLH conjugated synthetic peptide derived from human RNF43

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

RNF43 is a probable E3 ubiquitin-protein ligase that promotes cell growth and is upregulated in colon cancer. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Predicted Molecular Weight: 83


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ding, Meng. et al. Cancer-associated fibroblasts promote the stemness and progression of renal cell carcinoma via exosomal miR-181d-5p. CELL DEATH DISCOV. 2022 Nov;8(1):1-15Read more>>
VALIDATION IMAGES

Lane 1: Mouse Kidney lysates; Lane 2: Mouse Heart lysates probed with RNF43 Polyclonal Antibody, Unconjugated (bs-24331R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Pancreas tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Mouse Heart tissue lysates; Lane 5: Human 293T cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human A549 cell lysates; Lane 8: Human HL-60 cell lysates probed with RNF43 Polyclonal Antibody, Unconjugated (bs-24331R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.