bs-2425R [Primary Antibody]
Apelin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Apelin

Specificity: The antibody's immunogen sequence is derived from the Apelin-13 peptide. Therefore the Antibody can detect each of the active peptides that can be produced via proteolytic processing of the Apelin precursor.

Immunogen Range: 65-77/77


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8862

Swiss Prot: Q9ULZ1

Source: KLH conjugated synthetic peptide derived from human Apelin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 10


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kasacka et al. Comparative evaluation of cannabinoid receptors, apelin and S100A6 protein in the heart of women of different age groups. (2018) BMC.Cardiovasc.Disord. 18:190Read more>>
  • Cemre Nur Balci. et al. The investigation of apelin and apelin receptor expressions in mouse endometrium during peri-implantation period. ANN ANAT. 2023 Feb;246:152027Read more>>
  • Chen, Bin. et al. Apelin-13 Improves Cognitive Impairment and Repairs Hippocampal Neuronal Damage by Activating PGC-1/PPAR Signaling. NEUROCHEM RES. 2022 Dec;:1-12Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Apelin Polyclonal Antibody, Unconjugated (bs-2425R) at 1:400 overnight at 4°C, followed by secondary antibody, DAB, and counter-staining.