bs-2410R [Primary Antibody]
ABP/SHBG Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ABP/SHBG

Immunogen Range: 51-150/402


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6462

Swiss Prot: P04278

Source: KLH conjugated synthetic peptide derived from human ABP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 40


Cross Reactive Species: Mouse
Rat
Bovine

Predicted Cross Reactive Species: Human
Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Yaping, et al. "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." International Journal of Molecular Sciences 17.1 (2016): 96.Read more>>
  • Jiang Y et al. Transcriptome profile of bovine iPSCs derived from Sertoli Cells. Theriogenology. 2019 Nov 19. pii: S0093-691X(19)30522-9. Read more>>
  • Emi FUJII. et al. Morphological and biochemical characterization of Holstein cow skin at the tail root region susceptible to Chorioptes bovis and texanus parasitism. J VET MED SCI. 2024 08Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse uterus tissue labeled with Anti-ABP/SHBG Polyclonal Antibody, Unconjugated (bs-2410R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti ABP/SHBG Polyclonal Antibody, Unconjugated (bs-2410R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 44kD. Observed band size: 44kD.


Lane 1: Human HepG2 cell lysates; Lane 2: Human U87MG cell lysates probed with SHBG Polyclonal Antibody, Unconjugated (bs-2410R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.