DATASHEET
Host:
Rabbit
Target Protein:
Aldolase A, Aldolase B
Immunogen Range:
261-364/364
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
226
Swiss Prot:
P04075
Source:
KLH conjugated synthetic peptide derived from human Aldolase A
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).
PRODUCT SPECIFIC PUBLICATIONS
- Yao, Chun, et al. "Role of FADD Phosphorylation in Regulating Glucose Homeostasis: from Proteomic Discovery to Physiological Validation." Molecular & Cellular Proteomics (2013).Read more>>
- Xiang Yijia. et al. Scutellarin Protects against Myocardial Ischemiareperfusion Injury by Enhancing Aerobic Glycolysis through miR-34c-5p/ALDOA Axis. INT J APPL BASIC MED. 2024 May;14(2):85Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Aldolase Polyclonal Antibody, Unconjugated (bs-2406R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Muscle lysates probed with Aldolase A Polyclonal Antibody, Unconjugated (bs-2406R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Muscle tissue lysates; Lane 2: Rat Muscle tissue lysates probed with Aldolase A Polyclonal Antibody, Unconjugated (bs-2406R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.