Host: Rabbit
Target Protein: AKR1B1
Specificity: This antibody may have a secondary cross-reactivity with AKR1C1/2/3/4 due to a 75% sequence similarity in the corresponding immunogen regions.
Immunogen Range: 151-250/316
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 231
Swiss Prot: P15121
Source: KLH conjugated synthetic peptide derived from human AKR1B1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 35
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Luojie Liu. et al. Aberrant expression of AKR1B1 indicates poor prognosis and promotes gastric cancer progression by regulating the AKT-mTOR pathway. AGING-US. 2023 Sep 30; 15(18): 9661C9675Read more>>
Formalin-fixed and paraffin embedded rat tongue labeled with Anti-AKR1B1 Polyclonal Antibody, Unconjugated (bs-2405R) followed by conjugation to the secondary antibody
Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Adrenal gland tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human A549 cell lysates; Lane 7: Human U87MG cell lysates probed with AKR1B1 Polyclonal Antibody, Unconjugated (bs-2405R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.