DATASHEET
Host:
Rabbit
Target Protein:
KLF5
Immunogen Range:
61-160/457
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
688
Swiss Prot:
Q13887
Source:
KLH conjugated synthetic peptide derived from human KLF5
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The kinesin motor proteins include at least two forms of conventional kinesin encoded by different genes and designated as ubiquitous kinesin, which is expressed in all cells and tissues, or neuronal kinesin, which is expressed exclusively in neural cells. Kinesin is a microtubule associated protein comprised of three different structural domains. A considerable globular N-terminal domain regulates the hydrolysis of ATP and also microtubule binding while central coiled-coil domains promote heavy chain dimerization. Lastly, small globular C-terminal domains interact with kinesin light chains, membranous organelles and vesicles. Expression of ubiquitous kinesin heavy chain, also designated UKHC, is found subcellularly in areas of heavy vesicular trafficking such as the microtubule pathways of neural cells and also the Golgi of non-neural cell types.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D (2015) Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development. PLoS ONE 10(7): e0134390.Read more>>
VALIDATION IMAGES
L1 rat brain lysates L2 human colon carcinoma lysates probed with Anti KLF5/UKHC Polyclonal Antibody, Unconjugated (bs-2385R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 50kD. Observed band size:50kD.\n
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti KLF5/UKHC Polyclonal Antibody, Unconjugated (bs-2385R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-KLF5/UKHC Polyclonal Antibody, Unconjugated(bs-2385R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.