bs-2378R [Primary Antibody]
Notch 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Notch 2

Immunogen Range: 1501-1700/2471


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4853

Swiss Prot: Q04721

Source: KLH conjugated synthetic peptide derived from human Notch2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 262


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhan J et al. In Vivo Study on the Effects of Xiaoaiping on the Stemness of Hepatocellular Carcinoma Cells. Evid Based Complement Alternat Med. 2019 Jun 23;2019:4738243.Read more>>
  • Ying-Hui Zhou. et al. Notch1 is a marker for in situ resting osteocytes in a 3-dimensional gel culture model. CONNECT TISSUE RES. 2023 May 25Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Rabbit Anti-Notch 2 Polyclonal Antibody, Unconjugated (bs-2378R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Notch 2 Polyclonal Antibody, Unconjugated (bs-2378R) at 1:400 overnight at 4°C, DAB staining.