Host: Rabbit
Target Protein: Neutrophil Elastase
Immunogen Range: 51-150/265
Clonality: Polyclonal
Isotype: IgG
Swiss Prot: Q3UP87
Source: KLH conjugated synthetic peptide derived from human Neutrophil Elastase
Purification: Affinity purified by Protein A
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009].
Conjugation: AbBy Fluor® 750
Excitation/ Emission: 749nm/775nm
Size: 100ul
Concentration: 1ug/ul
Applications:
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100-500)
Predicted Molecular Weight: 26 kDa
Cross Reactive Species: Mouse
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.