bs-23426R [Primary Antibody]
PD-1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PD-1

Immunogen Range: 51-150/288


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5133

Swiss Prot: Q15116

Source: KLH conjugated synthetic peptide derived from human PD-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
CD279 is an immunoglobulin superfamily member, also known as Programmed Cell Death 1. Programmed Cell Death 1 is expressed on a subset of CD4-CD8 thymocytes, and on activated T and B cells. Programmed Cell Death 1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The Programmed Cell Death 1 ligands, PDL1 (also known as B7H1) and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 30


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela Cell lysates; Lane 2: Molt-4 Cell lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-23426R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Raji Cell lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-23426R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Jurkat Cell lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-23426R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.