bs-23303R [Primary Antibody]
NFKB p65 (Ser311) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKB p65 (Ser311)

Modification Site: Ser311

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5970

Swiss Prot: Q04206

Source: KLH conjugated synthesised phosphopeptide derived from human NFKB p65 around the phosphorylation site of Ser311

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()

Predicted Molecular Weight: 61 kDa


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;()Read more>>
  • Jiaqi Li. et al. Gold Nanoparticle Delivery of Glut1 siRNA Facilitates Glucose Starvation Therapy in Lung Cancer. CHEMBIOCHEM. 2024 Apr;:e202400239Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 (Ser311) Polyclonal Antibody, Unconjugated (bs-23303R) at 1:200 overnight at 4°C, DAB staining.