bs-23217R [Primary Antibody]
NFKB p65 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKB p65

Immunogen Range: 301-320/551


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5970

Swiss Prot: Q04206

Source: KLH conjugated synthetic peptide derived from human NFKB p65

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen X et al. Hesperetin derivative attenuates CCl4-induced hepatic fibrosis and inflammation by Gli-1-dependent mechanisms. Int Immunopharmacol. 2019 Aug 29;76:105838. Read more>>
  • Ji-Sheng Wang. et al. Effect of leech-centipede medicine on improving erectile function in DIED rats via PKC signalling pathway-related molecules. J Ethnopharmacol. 2021 Mar;267:113463Read more>>
  • Xiao Li. et al. Mechanism by which Huoxue Tongluo Qiwei Decoction improves the erectile function of rats with diabetic erectile dysfunction. J Ethnopharmacol. 2022 Jan;283:114674Read more>>
  • Linyan Cheng. et al. Icariin attenuates thioacetamide_induced bone loss via the RANKL_p38/ERK_NFAT signaling pathway. Mol Med Rep. 2022 Apr;25(4):1-11Read more>>
  • Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635Read more>>
  • Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;()Read more>>
  • Pu, Luya. et al. Laminar shear stress alleviates monocyte adhesion and atherosclerosis development via miR-29b-3p/CX3CL1 axis regulation. J CELL SCI. 2022 Jul;135(14)Read more>>
  • Chen Bai. et al. Ac2?26 alleviates hepatic ischemia?reperfusion injury based on inhibiting the positive feedback loop of HMGB1/TLR4/NF?B/neutrophils. EXP THER MED. 2022 Nov;24(5):1-1Read more>>
  • Yiming Bi. et al. -Sitosterol Suppresses LPS-Induced Cytokine Production in Human Umbilical Vein Endothelial Cells via MAPKs and NF-<i></i>B Signaling Pathway. EVID-BASED COMPL ALT. 2023 Jan 03;2023:924109Read more>>
  • Congcong Chen. et al. Radix Paeoniae Alba attenuates Radix Bupleuri-induced hepatotoxicity by modulating gut microbiota to alleviate the inhibition of saikosaponins on glutathione synthetase. J PHARM ANAL. 2023 ApRead more>>
  • Zaozao Pan. et al. Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells. INT J MOL SCI. 2023 Jan;24(8):7242Read more>>
  • Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
  • Zhuang Cuicui. et al. Lycopene promoted M2 macrophage polarization via inhibition of NOTCH1-PI3K-mTOR-NF-B-JMJD3-IRF4 pathway in response to Escherichia coli infection in J744A.1 cells. ARCH MICROBIOL. 2024 Jun;206(6):1-11Read more>>
VALIDATION IMAGES

Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human HepG2 cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-23217R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.