bs-23063R
[Primary Antibody]
Vimentin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Vimentin
Immunogen Range: 101-200/466
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7431
Swiss Prot: P08670
Source: KLH conjugated synthetic peptide derived from human Vimentin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 53
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Hou L et al. Nontoxic concentration of ochratoxin A decreases the dosage of cyclosporine A to induce chronic nephropathy model via autophagy mediated by toll-like receptor 4. Cell Death Dis. 2020 Feb 27;11(2):153. Read more>>
- Hou L et al. Nontoxic Concentration of Ochratoxin A Decreases the Dosage of Cyclosporine A to Induce Chronic Nephropathy Model via Autophagy Mediated by Toll-Like Receptor 4. Cell Death Dis. 2020 Feb 27;11(2):153.Read more>>
- Huimeng Qi. et al. MicroRNA-183 exerts a protective role in lupus nephritis through blunting the activation of TGF-/Smad/TLR3 pathway via reducing Tgfbr1. Exp Cell Res. 2020 Sep;394:112138Read more>>
- Huajun Wang. et al. LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis. Cancer Cell Int. 2020 Dec;20(1):1-12Read more>>
- Xinru Mao. et al. Selenomethionine alleviated Ochratoxin A induced pyroptosis and renal fibrotic factors expressions in MDCK cells. 2021 Oct 21Read more>>
- Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041Read more>>
- Honghua Yue. et al. Colchicine: Emerging therapeutic effects on atrial fibrillation by alleviating myocardial fibrosis in a rat model. BIOMED PHARMACOTHER. 2022 Oct;154:113573Read more>>
- Heng Du. et al. Nontoxic Concentration of Ochratoxin A Aggravates Renal Fibrosis Induced by Adriamycin/Cyclosporine A Nephropathy via TGF-1/SMAD2/3. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
- Jiajia Xu. et al. Therapeutic function of a novel rat induced pluripotent stem cell line in a 6?OHDA?induced rat model of Parkinson's disease. INT J MOL MED. 2022 Dec;50(6):1-13Read more>>
- Xiaoyan Song. et al. SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway. TISSUE CELL. 2022 Oct;:101954Read more>>
- Yunan Wang. et al. A novel type of mesenchymal stem cells derived from bovine metanephric mesenchyme. TISSUE CELL. 2022 Dec;79:10197Read more>>
- Junyuan Ma. et al. Effects of melatonin on development and hormone secretion of sheep theca cells in?vitro. THERIOGENOLOGY. 2023 Mar;198:172Read more>>
- Jill A. Poole. et al. Combined repetitive inhalant endotoxin and collagen-induced arthritis drives inflammatory lung disease and arthritis severity in a testosterone-dependent manner. AM J PHYSIOL-LUNG C. 2023 Dec;(Articles in Press)Read more>>
- Qingbo Zheng. et al. Interpretation of the Yak Skin Single-Cell Transcriptome Landscape. ANIMALS. 2023 Jan;13(24):3818Read more>>
- Haoyuan Wang. et al. Human amniotic mesenchymal stem cells inhibit immune rejection injury from allogeneic mouse heart transplantation: A preliminary study on the microRNA expression. TRANSPL IMMUNOL. 2024 Jun;84:102022Read more>>
- Xin Bin. et al. circMTO1/miR-30c-5p/SOCS3 axis alleviates oral submucous fibrosis through inhibiting fibroblastmyofibroblast transition. J ORAL PATHOL MED. 2024 MayRead more>>
- Shouying Xu. et al. ARID1A restrains EMT and stemness of ovarian cancer cells through the Hippo pathway. INT J ONCOL. 2024 Aug;65(2):1-11Read more>>
- Zhou Guanglei. et al. Cytotoxicity and cell migration evaluation of a strontium silicate-based root canal sealer on stem cells from rat apical papilla: an in vitro study. BMC ORAL HEALTH. 2024 Dec;24(1):1-1Read more>>
VALIDATION IMAGES
Lane 1: U2OS Cell lysates; Lane 2: U87MG Cell lysates; Lane 3: HeLa lysates; Lane 4: Jurkat lysates; probed with Vimentin Polyclonal Antibody, unconjugated (bs-23063R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human cervical cancer section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-23063R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Hela Cell lysate; Probed with Vimentin Polyclonal Antibody, Unconjugated (bs-23063R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) polyclonal Antibody, Unconjugated (bs-23063R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
25 ug total protein per lane of various lysates (see on figure) probed with Vimentin polyclonal antibody, unconjugated (bs-23063R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Vimentin polyclonal antibody, unconjugated (bs-23063R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.