Host: Rabbit
Target Protein: ACE2
Immunogen Range: 251-350/805
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 59272
Swiss Prot: Q9BYF1
Source: KLH conjugated synthetic peptide derived from human ACE2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 87
Cross Reactive Species:
Human
Rat
Bacteria
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Rat Ovary lysates; Lane 2:SH-SY5Y cell lysates; Lane 3:HepG2 cell lysates; Lane 4: A431 cell lysates probed with ACE2 Polyclonal Antibody, Unconjugated (bs-23027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: ACE2 Protein Overexpression E.coli lysates; Lane 2: Control group E.coli Lysates probed with ACE2 Polyclonal Antibody, Unconjugated (bs-23027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ACE2 Polyclonal Antibody(bs-23027R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).