bs-22924R [Primary Antibody]
iNOS Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: iNOS

Immunogen Range: 811-910/1153


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18126

Swiss Prot: P29477

Source: KLH conjugated synthetic peptide derived from mouse iNOS

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(IHC-P=1:400-800)
IHC-F(IHC-F=1:400-800)
IF(IF=1:100-500)

Predicted Molecular Weight: 130


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Mouse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Uygar Sa??k. et al. Effect of ferulic acid on testicular damage caused by torsion-detorsion in rats. BIOTECH HISTOCHEM. 2022 Aug 23Read more>>
  • Xiao-Dan Li. et al. Fabricating biodegradable calcium phosphate/calcium sulfate cement reinforced with cellulose: in vitro and in vivo studies. J MATER CHEM B. 2022 NovRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with iNOS Polyclonal Antibody, Unconjugated (bs-22924R) at 1:200 overnight at 4°C, DAB staining.