bs-22897R [Primary Antibody]
ATG13 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ATG13

Immunogen Range: 41-140/517


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9776

Swiss Prot: O75143

Source: KLH conjugated synthetic peptide derived from human ATG13

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)

Predicted Molecular Weight: 57


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Sheep
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yu Zhang. et al. FoxO1 silencing in Atp7b/ neural stem cells attenuates high copper-induced apoptosis via regulation of autophagy. J NEUROCHEM. 2024 JuRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATG13 Polyclonal Antibody, Unconjugated (bs-22897R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATG13 Polyclonal Antibody, Unconjugated (bs-22897R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATG13 Polyclonal Antibody, Unconjugated (bs-22897R) at 1:200 overnight at 4°C, DAB staining.