bs-22884R [Primary Antibody]
phospho-SNAIL + SLUG (Ser246) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: phospho-SNAIL + SLUG (Ser246)

Modification Site: Ser246

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6615

Swiss Prot: O95863

Source: KLH conjugated synthetic peptide derived from human phospho-SNAIL + SLUG (Ser246)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm. Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with phospho-SNAIL + SLUG (Ser246) Polyclonal Antibody, Unconjugated (bs-22884R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with phospho-SNAIL + SLUG (Ser246) Polyclonal Antibody, Unconjugated (bs-22884R) at 1:200 overnight at 4°C, DAB staining.