Host: Rabbit
Target Protein: Nur77
Immunogen Range: 101-200/598
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3164
Swiss Prot: P22736
Source: KLH conjugated synthetic peptide derived from human Nur77
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()
ICC(1:100-500)
Predicted Molecular Weight: 66
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nur77 Polyclonal Antibody, Unconjugated (bs-22601R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nur77 Polyclonal Antibody, Unconjugated (bs-22601R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nur77 Polyclonal Antibody, Unconjugated (bs-22601R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nur77 Polyclonal Antibody, Unconjugated (bs-22601R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with Nur77 Polyclonal Antibody, Unconjugated (bs-22601R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.