Host: Rabbit
Target Protein: SIRT1/sirtuin 1
Immunogen Range: 551-650/747
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 23411
Swiss Prot: Q96EB6
Source: KLH conjugated synthetic peptide derived from human SIRT1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 81
Cross Reactive Species:
Human
Mouse
Dog
Predicted Cross Reactive Species:
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- "Liu GZ et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model.Naunyn Schmiedebergs Arch Pharmacol. 2019 Mar 9."Read more>>
- He Zhang. et al. Feeding frequency affects glucose and lipid metabolism through SIRT1/AMPK pathway in growing pigs with the same amount of daily feed. J Nutr Biochem. 2021 Nov;:108919Read more>>
- Wan Boyang. et al. Zearalenone promotes follicle development through activating SIRT1/PGC-1_ signaling pathway in the ovaries of weaned gilts. J Anim Sci. 2022 FebRead more>>
- Cuiting Liao. et al. Inhibition of NAD kinase elevates the hepatic NAD+ pool and alleviates acetaminophen-induced acute liver injury in mice. BIOCHEM BIOPH RES CO. 2022 Jul;612:7Read more>>
- Yu Wang. et al. Seabuckthorn Reverses High-Fat-Diet-Induced Obesity and Enhances Fat Browning via Activation of AMPK/SIRT1 Pathway. NUTRIENTS. 2022 Jan;14(14):2903Read more>>
- Wang, Daquan. et al. Protection of zero-valent iron nanoparticles against sepsis and septic heart failure. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22Read more>>
- Hongye Liu. et al. The ginsenoside Rh2 protects porcine oocytes against aging and oxidative stress by regulating SIRT1 expression and mitochondrial activity. THERIOGENOLOGY. 2023 Apr;200:125Read more>>
- Kaihui Xie. et al. Overexpression of SIRT1 alleviates oxidative damage and barrier dysfunction in CPB2 toxin-infected IPEC-J2 cells. MICROB PATHOGENESIS. 2023 Jun;:106181Read more>>
- Lijuan Bao. et al. Hexadecanamide alleviates Staphylococcus aureus-induced mastitis in mice by inhibiting inflammatory responses and restoring blood-milk barrier integrity. PLOS PATHOG. 2023 Nov;19(11):e1011764Read more>>
- Wangrui Lei. et al. Isopropyl 3-(3,4-dihydroxyphenyl) 2-hydroxypropanoate protects septic myocardial injury via regulating GAS6/Axl-AMPK signaling pathway. BIOCHEM PHARMACOL. 2024 Feb;:116035Read more>>
- Mingran Dong. et al. Anthocyanins from Lycium ruthenicum Murray Mitigate Cadmium-Induced Oxidative Stress and Testicular Toxicity by Activating the Keap1/Nrf2 Signaling Pathway. PHARMACEUTICALS-BASE. 2024 Mar;17(3):322Read more>>
- Mei Zhang. et al. Protective Effects of Resveratrol on Cytotoxicity of Mouse Hepatic Stellate Cells Induced by PM2.5. ATMOSPHERE-BASEL. 2024 May;15(5):588Read more>>
Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-SIRT1 Polyclonal Antibody, Unconjugated (bs-2257R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SIRT1/sirtuin 1 Polyclonal Antibody, Unconjugated (bs-2257R) at 1:400 overnight at 4°C, DAB staining.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SIRT1 Polyclonal Antibody(bs-2257R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Hela cell lysates; Lane 2: NIH/3T3 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: 293T cell lysates ; Lane 5: Mouse Testis lysates probed with SIRT1/sirtuin 1 Polyclonal Antibody, Unconjugated (bs-2257R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Mouse Testis lysates probed with SIRT1/sirtuin 1 Polyclonal Antibody, Unconjugated (bs-2257R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.