bs-2252R [Primary Antibody]
GABA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GABA

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated GABA

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 0.1031


Cross Reactive Species: Rat
Others
(GABA)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Luo, Li, et al. "Niche astrocytes promote the survival, proliferation and neuronal differentiation of co‑transplanted neural stem cells following ischemic stroke in rats." Experimental and Therapeutic Medicine.Read more>>
  • Li-Min Zhang. et al. Subanesthetic dose of S-ketamine improved cognitive dysfunction via the inhibition of hippocampal astrocytosis in a mouse model of post-stroke chronic stress. J PSYCHIATR RES. 2023 Feb;158:1Read more>>
  • Wang Lu-Ying. et al. NLRP3CGABA signaling pathway contributes to the pathogenesis of impulsive-like behaviors and cognitive deficits in aged mice. J NEUROINFLAMM. 2023 Dec;20(1):1-15Read more>>
  • Taotao Li. et al. Rosemary (Rosmarinus officinalis L.) hydrosol based on serotonergic synapse for insomnia. J ETHNOPHARMACOL. 2023 Jul;:116984Read more>>
  • Dong-Xue Zhang. et al. Icariin mitigates anxiety-like behaviors induced by hemorrhagic shock and resuscitation via inhibiting of astrocytic activation. PHYTOMEDICINE. 2024 Jun;128:155507Read more>>
VALIDATION IMAGES

Formalin-fixed and rat brain tissue labeled with Anti-GABA Polyclonal Antibody, Unconjugated (bs-2252R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GABA Polyclonal Antibody, Unconjugated (bs-2252R) at 1:200 overnight at 4°C, DAB staining.