bs-2225R-FITC [Conjugated Primary Antibody]
Smad3 (Ser423) Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Smad3 Ser423

Modification Site: Ser423

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4088

Source: KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
  • Han B et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2019 Dec 23;259:113870.Read more>>
  • Keiko M et al. Cultured Neonatal Rat Cardiomyocytes Continue Beating Through Upregulation of CTGF Gene ExpressionJ Nippon Med Sch.2020;87(5):268-276.Read more>>
  • Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
  • Tashiro Kensuke. et al. Extracellular vesicles from adipose-derived stem cells relieve extremity lymphedema in mouse models.. PLAST RECONSTR SURG. 2023 Mar;:10.1097/PRS.0000000000010388Read more>>
  • Xiaoliang Zhou. et al. Ursolic acid inhibits human dermal fibroblasts hyperproliferation, migration, and collagen deposition induced by TGF- via regulating the Smad2/3 pathway. GENE. 2023 May;867:147367Read more>>
VALIDATION IMAGES