Host: Rabbit
Target Protein: Smad3 Ser423
Modification Site: Ser423
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4088
Source: KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species: Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
- Han B et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2019 Dec 23;259:113870.Read more>>
- Keiko M et al. Cultured Neonatal Rat Cardiomyocytes Continue Beating Through Upregulation of CTGF Gene ExpressionJ Nippon Med Sch.2020;87(5):268-276.Read more>>
- Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
- Tashiro Kensuke. et al. Extracellular vesicles from adipose-derived stem cells relieve extremity lymphedema in mouse models.. PLAST RECONSTR SURG. 2023 Mar;:10.1097/PRS.0000000000010388Read more>>
- Xiaoliang Zhou. et al. Ursolic acid inhibits human dermal fibroblasts hyperproliferation, migration, and collagen deposition induced by TGF- via regulating the Smad2/3 pathway. GENE. 2023 May;867:147367Read more>>
Human A549 lysates probed with p-Smad3(Ser423) Polyclonal Antibody, Unconjugated (bs-2225R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad3 (Ser423) Polyclonal Antibody(bs-2225R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Heart lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Cerebrum lysates probed with Smad3 Polyclonal Antibody, Unconjugated (bs-2225R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.