bs-2211R
[Primary Antibody]
CD80 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD80
Immunogen Range: 251-321/321
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 25408
Swiss Prot: G3V671
Source: KLH conjugated synthetic peptide derived from rat CD80
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 30
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chen, Quan, et al. "Prevention of ultraviolet radiation‑induced immunosuppression by sunscreen in Candida albicans‑induced delayed‑type hypersensitivity." Molecular Medicine Reports (2016).Read more>>
- Ju, Huanyu, et al. "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." BMC immunology 17.1 (2016): 31.Read more>>
- Liang, Zhuoping, et al. "Role of dendritic cells in consistency between upper and lower airway inflammatory responses: animal experimental studies." Int J Clin Exp Pathol 9.10 (2016): 10093-10104.Read more>>
- Han, Sheng, et al. "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83.Read more>>
- da Silva Junior, Evaldo Moreira, et al. "Modulating effect of low intensity pulsed ultrasound on the phenotype of inflammatory cells." Biomedicine & Pharmacotherapy (2017).Read more>>
- Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31Read more>>
- Yu S et al. Dendritic cells modified with Der p1 antigen as a therapeutic potential for allergic rhinitis in a murine model via regulatory effects on IL-4, IL-10 and IL-13.Int Immunopharmacol. 2019 May;70:216-224. Read more>>
- Moser PT et al. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue Eng Part A. 2019 Oct 30. Read more>>
- Leiblein M et al. Size matters: Effect of granule size of the bone graft substitute (Herafill®) on bone healing using Masquelet's induced membrane in a critical size defect model in the rat's femur. J Biomed Mater Res B Appl Biomater. 2019 Nov 13.Read more>>
- Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110. Read more>>
- Bai J et al. Reactive Oxygen Species‐Scavenging Scaffold with Rapamycin for Treatment of Intervertebral Disk Degeneration. Adv Healthc Mater. 2019 Dec 10:e1901186.Read more>>
- Li TF et al. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma.Biomaterials. 2018 Oct;181:35-52.Read more>>
- Philipp T Moser et al. Creation of Laryngeal Grafts from Primary Human Cells and Decellularized Laryngeal Scaffolds. Tissue Eng Part A. 2020 May;26(9-10):543-555.Read more>>
- Lidija Gradi?nik. et al. Isolation, characterisation and phagocytic function of human macrophages from human peripheral blood. Mol Biol Rep. 2020 Sep;47(9):6929-694Read more>>
- Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306Read more>>
- Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211Read more>>
- Xuan Wang. et al. Probiotic Bifidobacterium bifidum G9-1 Has a Preventive Effect on the Acceleration of Colonic Permeability and M1 Macrophage Population in Maternally Separated Rats. Biomedicines. 2021 Jun;9(6):641Read more>>
- Xu, Hua-Zhen. et al. Synergy of nanodiamondCdoxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells. J Nanobiotechnol. 2021 Dec;19(1):1-24Read more>>
- Masataka Kadowaki. et al. Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation. LIFE-BASEL. 2022 Nov;12(11):1812Read more>>
- Linghao Xiao. et al. Osteogenic capacity of octacalcium phosphate involving macrophage polarization. J BIOMED MATER RES A. 2022 DecRead more>>
- Maria Apriliani Gani. et al. Bovine hydroxyapatite-based scaffold accelerated the inflammatory phase and bone growth in rats with bone defect. J APPL BIOMATER FUNC. ;()Read more>>
- Tianrui Zhang. et al. Daphnetin Improves Neuropathic Pain by Inhibiting the Expression of Chemokines and Inflammatory Factors in the Spinal Cord and Interfering with Glial Cell Polarization. PHARMACEUTICALS-BASE. 2023 Feb;16(2):243Read more>>
- Yanlun Zhu. et al. Oral Delivery of Bioactive Glass-Loaded CoreCShell Hydrogel Microspheres for Effective Treatment of Inflammatory Bowel Disease. Advanced Science. 2023 Apr;:2207418Read more>>
- Gaolong Lin. et al. Polydopamine-cladded montmorillonite micro-sheets as therapeutic platform repair the gut mucosal barrier of murine colitis through inhibiting oxidative stress. MATER TODAY BIO. 2023 Jun;20:100654Read more>>
- Xing Chang. et al. Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT. BIOCHEM PHARMACOL. 2023 May;:115582Read more>>
- Zhejie Chen. et al. Oral Delivery of Transformable Bilirubin Self-Assembled System for Targeted Therapy of Colitis. ADV HEALTHC MATER. 2023 Jun;:2300946Read more>>
- Shu Watanabe. et al. Knee Osteotomy Decreases Joint Inflammation Based on Synovial Histology and Synovial Fluid Analysis. ARTHROSCOPY. 2023 JulRead more>>
- Cui Tang. et al. CpG-Conjugated Silver Nanoparticles as a Multifunctional Nanomedicine to Promote Macrophage Efferocytosis and Repolarization for Atherosclerosis Therapy. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Wang Chao. et al. PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer. J NANOBIOTECHNOL. 2023 Dec;21(1):1-21Read more>>
- Chen Xiaolin. et al. Dapagliflozin ameliorates ovulation disorders via attenuating activated microglia-mediated hypothalamic inflammation in HFD-fed mice. NEUROENDOCRINOLOGY. 2023 Dec;:1-1Read more>>
- Xu Hua-Zhen. et al. Anti-lung cancer synergy of low-dose doxorubicin and PD-L1 blocker co-delivered via mild photothermia-responsive black phosphorus. DRUG DELIV TRANSL RE. 2024 Apr;:1-22Read more>>
- Han Duan. et al. Hepatocyte growth factor enhances the ability of dental pulp stem cells to ameliorate atherosclerosis in apolipoprotein E-knockout mice. WORLD J STEM CELLS. 2024 May 26; 16(5): 57559Read more>>
- Kunjie Ran. et al. Sustained-release of SOD from multivesicular liposomes accelerated the colonic mucosal healing of colitis mice by inhibiting oxidative stress. COLLOID SURFACE B. 2024 Aug;:114143Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-B7-1/CD80 Polyclonal Antibody, Unconjugated (bs-2211R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-2211R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Raw264.7 cell lysates; Lane 2: Mouse SP2/0 cell lysates; Lane 3: Rat Thymus tissue Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human K562 cell Lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-2211R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.