bs-2210R-Cy3 [Conjugated Primary Antibody]
p38 MAPK (Thr180 + Tyr182) Antibody, Cy3 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: p38 MAPK Thr180 + Tyr182

Modification Site: Thr180 + Tyr182

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1432

Swiss Prot: Q16539

Source: KLH conjugated synthetic phosphopeptide derived from human p38 MAPK around the phosphorylation site of(Thr180/Tyr182)

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat
Chicken
Goat

Predicted Cross Reactive Species: Dog
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Iriyama, Noriyoshi, Yoshihiro Hatta, and Masami Takei. "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." Cancer Medicine (2016).Read more>>
  • Iriyama et al. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. (2016) Cancer.Med. 5:3223-3234Read more>>
  • Wang et al. Upregulation of progranulin by Helicobacter pylori in human gastric epithelial cells via p38MAPK and MEK1/2 signaling pathway: role in epithelial cell proliferation and migration. (2011) FEMS.Immunol.Med.Microbiol. 63:82-92Read more>>
  • Kishi et al. Pro-metastatic signaling of the trans fatty acid elaidic acid is associated with lipid rafts. (2018) Oncol.Lett. 15:4423-4426Read more>>
  • Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193.Read more>>
  • Li QH et al. Effect of heat stress on mitogen-activated protein kinases in the hypothalamic− pituitary− gonadal axis of developing Wenchang chicks. Poultry Science.2019.Read more>>
  • Hung YY et al. Deficiency in Androgen Receptor Aggravates the Depressive-Like Behaviors in Chronic Mild Stress Model of Depression. Cells. 2019 Sep 2;8(9). pii: E1021.Read more>>
  • Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
  • Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
  • Liu A et al. Catalpol ameliorates psoriasis-like phenotypes via SIRT1 mediated suppression of NF-B and MAPKs signaling pathwaysBioengineered.2021 Dec;12(1):183-195.Read more>>
  • Li X et al. Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites. Cell Rep. 2020 Jun 16;31(11):107765.Read more>>
  • Wenbo Ge. et al. 17-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro. Mol Immunol. 2020 Nov;127:21Read more>>
  • Ting Zhang. et al. Sea buckthorn ( Hippophae rhamnoides L.) oil enhances proliferation, adipocytes differentiation and insulin sensitivity in 3T3-L1 cells. Food Sci Biotechnol. 2020 Nov;29(11):1511-1518Read more>>
  • Chinthalapally V. Rao. et al. GSK3\ARC/Arg3.1 and GSK3\Wnt signaling axes trigger amyloid\ accumulation and neuroinflammation in middle\aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221Read more>>
  • Shenjie Zhong. et al. Bmp8a is an essential positive regulator of antiviral immunity in zebrafish. Commun Biol. 2021 Mar;4(1):1-15Read more>>
  • Xiangdong Meng. et al. Naringin ameliorates memory deficits and exerts neuroprotective effects in a mouse model of Alzheimer's disease by regulating multiple metabolic pathways. Mol Med Rep. 2021 May;23(5):1-13Read more>>
  • Meiqiong Wu. et al. Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway. SCI TOTAL ENVIRON. 2022 Apr;:155558Read more>>
  • Liu Yang. et al. Protective effect of isopsoralen on UVB-induced injury in HaCaT cells via the ER and p38MAPK signaling pathways. J FOOD BIOCHEM. 2022 Apr 12Read more>>
  • L Cheng. et al. Toxic effects of thioacetamide-induced femoral damage in New Zealand white rabbits by activating the p38/ERK signaling pathway. PHYSIOL RES. 2022 Apr 11Read more>>
  • Zhaomin Zheng. et al. New insight into the structure-dependent two-way immunomodulatory effects of water-soluble yeast _-glucan in macrophages. CARBOHYD POLYM. 2022 Sep;291:119569Read more>>
  • Shuting Wei. et al. Particle matters induce airway epithelial barrier dysfunction in vivo and in vitro: from a more realistic inhalation scenario. ENVIRON SCI-NANO. 2022 JuRead more>>
  • Wen, Yukang. et al. Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages. VET RES. 2022 Dec;53(1):1-15Read more>>
  • Li, Xiao. et al. Synergistic neuroprotective effect of saikosaponin A and albiflorin on corticosterone-induced apoptosis in PC12 cells via regulation of metabolic disorders and neuroinflammation. MOL BIOL REP. 2022 Aug;:1-13Read more>>
  • Yanjie Wang. et al. Inhibitory Effect of Bisdemethoxycurcumin on DNCB-Induced Atopic Dermatitis in Mice. MOLECULES. 2023 Jan;28(1):293Read more>>
  • Lin, Xiaoxin. et al. Shikonin promotes rat periodontal bone defect repair and osteogenic differentiation of BMSCs by p38 MAPK pathway. ODONTOLOGY. 2022 Dec;:1-9Read more>>
  • Manman Shen. et al. Effects of quercetin on granulosa cells from pre-hierarchical follicles by modulating MAPK signaling pathway in chicken. POULTRY SCIENCE. 2023 Apr;:102736Read more>>
  • Lin Niu. et al. Magnolol alleviates pulmonary fibrosis inchronic obstructive pulmonary disease by targeting transient receptor potential vanilloid 4-ankyrin repeat domain. PHYTOTHER RES. 2023 JuRead more>>
  • Zhong Shenjie. et al. Bmp8a deletion leads to obesity through regulation of lipid metabolism and adipocyte differentiation. COMMUN BIOL. 2023 Aug;6(1):1-13Read more>>
  • Lihui Chen. et al. Bmp4 in Zebrafish Enhances Antiviral Innate Immunity through p38 MAPK (Mitogen-Activated Protein Kinases) Pathway. INT J MOL SCI. 2023 Jan;24(19):14444Read more>>
  • Zhao Xu. et al. Sirt1 inhibits macrophage polarization and inflammation in gouty arthritis by inhibiting the MAPK/NF-B/AP-1 pathway and activating the Nrf2/HO-1 pathway. INFLAMM RES. 2024 May;:1-12Read more>>
  • Chenhao Xu. et al. Human umbilical cord mesenchymal stem cells alleviate fatty liver ischemia-reperfusion injury by activating autophagy through upregulation of IFN. CELL BIOCHEM FUNCT. 2024 Jun;42(4):e404Read more>>
  • Yuan Yan. et al. Study on the mechanism of heterogeneous tumor-associated macrophages in three subtypes of breast cancer through the integration of single-cell RNA sequencing and in vitro experiments. MOL BIOL REP. 2024 Dec;51(1):1-16Read more>>
  • Yuquan Guo. et al. Revealing the Mechanism: The Influence of Baicalin on M1/M2 and Th1/Th2 Imbalances in Mycoplasma gallisepticum Infection. POULTRY SCI. 2024 Jul;:104145Read more>>
VALIDATION IMAGES