bs-2202R [Primary Antibody]
VEGFR3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VEGFR3

Immunogen Range: 901-1000/1298


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2324

Swiss Prot: P35916

Source: KLH conjugated synthetic peptide derived from Mouse VEGFR-3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 151


Cross Reactive Species: Human
Mouse
Bovine

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhuo, Wei, et al. "The CXCL12?CCXCR4 Chemokine Pathway: A Novel Axis Regulates Lymphangiogenesis."Clinical Cancer Research 18.19 (2012): 5387-5398.lRead more>>
  • Wang, Zheng, et al. "RhGH attenuates ischemia injury of intrahepatic bile ducts relating to liver transplantation." Journal of Surgical Research 171.1 (2011): 300-310.Read more>>
  • Wang, Zhixiong, et al. "CXCL1 from tumor-associated lymphatic endothelial cells drives gastric cancer cell into lymphatic system via activating integrin β1/FAK/AKT signaling." Cancer Letters 385 (2017): 28-38.Read more>>
  • Hayashi KG et al. Temporal expression and localization of vascular endothelial growth factor family members in the bovine uterus during peri-implantation period. Theriogenology. 2019 Apr 24;133:56-64.Read more>>
  • M Cs?b?nyeiov. et al. Immunohistochemical and Scanning Electron Microscopic Confirmation of the Lymphatic Lacunae in the Uterine Tube Mucosal Folds. What Are the Clinical Implications?. PHYSIOL RES. 2022 Dec 27;71(Suppl 1):S115-S123Read more>>
VALIDATION IMAGES

A549 cells probed with \tVEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded Human Gastric Cancer labeled with VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded Human Gastric Cancer labeled with VEGFR3 Polyclonal Antibody, Unconjugated (bs-2202R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VEGFR3) Polyclonal Antibody, Unconjugated (bbs-2202R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.