bs-2194R [Primary Antibody]
EGFP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EGFP

Specificity: This antibody will work best with EGFP that has a similar amino acid make-up as that of Aequorea Victoria.

Immunogen Range: 1-239/239


Clonality: Polyclonal

Isotype: IgG

Source: Purified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 27


Cross Reactive Species: Human
Rat
Tags
(EGFP protein)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Peng, Xu, et al. "Identification of ITGB4BP as a new interaction protein of P311." Life Sciences (2012).Read more>>
  • Yoshida, Shuhei, et al. "Adipose-derived stem cell transplantation for therapeutic lymphangiogenesis in a mouse secondary lymphedema model."Regenerative medicine 10.5 (2015): 549-562.Read more>>
  • Xie, Teng, et al. "Is X-linked methyl-CpG binding protein 2 a new target for the treatment of Parkinson's disease." Neural Regeneration Research 8.21 (2013): 1948.Read more>>
  • Feng S et al. Development of a reverse genetics system for snakehead vesiculovirus (SHVV). (2018) Virology. Oct 15;526:32-37. Read more>>
  • Feng S et al. Development of a reverse genetics system for snakehead vesiculovirus (SHVV). (2018) Virology. Oct 15;526:32-37. Read more>>
  • Yang Sidi. et al. Salmonella Effector SpvB Inhibits NF-B Activity via KEAP1-Mediated Downregulation of IKK. Front Cell Infect Mi. 2021 Mar;11:207Read more>>
  • Yongsen Wen. et al. Construction and immune evaluation of the recombinant duck adenovirus type 3 delivering capsid protein VP1 of the type 1 duck hepatitis virus. POULTRY SCIENCE. 2023 Sep;:103117Read more>>
  • Haimei Qin. et al. Establishing an induced infertile chicken line for efficient germline transmission of exogenous PGCs. J INTEGR AGR. 2024 AugRead more>>
VALIDATION IMAGES

Transfected HBX cells probed with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Formalin-fixed and paraffin embedded rat liver labeled with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 followed by conjugation to the secondary antibody


Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P) probed with EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.