Host: Rabbit
Target Protein: Uromodulin
Immunogen Range: 351-450/642
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7369
Swiss Prot: P07911
Source: KLH conjugated synthetic peptide derived from Human Uromodulin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 61/65
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Cow
For research use only. Not intended for diagnostic or therapeutic use.
- Kim et al. Blocking junctional adhesion molecule C promotes the recovery of cisplatin-induced acute kidney injury. (2017) Korean.J.Intern.Me. 32:1053-1061Read more>>
- Cho, Wonyong, et al. "Fate of Neutrophils during the Recovery Phase of Ischemia/Reperfusion Induced Acute Kidney Injury." Journal of Korean Medical Science 32.10 (2017): 1616-1625.Read more>>
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-MCKD2/UMOD Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Rabbit Anti-Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Lane 1: Rat Kidney lysates; Lane 2: Human Kidney Carcinoma lysates; Probed with Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:300, overnight at 4˚C. Followed by secondary antibody (bs-0295G-HRP) at 1:3000 for 90min at RT.
Mouse kidney cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Blocking was performed with 1 X PBS containing 0.5% BSA + 10% goat serum for 1 hour. Uromodulin Polyclonal Antibody (bs-2189R, 1μg/1x10^6 cells) was incubated with the cells for 30 min at room temperature. Then a Goat Anti-rabbit IgG/FITC (bs-0295G-FITC) secondary antibody was added at 1:200 for 40 mins at room temperature. The graph compares primary antibody (green) to unstained cells (dark blue), secondary only (light blue) and isotype control (orange; bs-0295P).
Paraformaldehyde-fixed, paraffin-embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 overnight at 4°C. A Goat Anti-Rabbit IgG, FITC Conjugated secondary antibody (bs-0295G-FITC) was used for 40 mins at 37°C. DAPI (5ug/ml, blue) was used for nuclei staining.
Paraformaldehyde-fixed, paraffin-embedded Rat Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 overnight at 4°C. A Goat Anti-Rabbit IgG, Cy3 Conjugated secondary antibody (bs-0295G-Cy3) was used for 40 mins at 37°C. DAPI (5ug/ml, blue) was used for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Uromodulin) Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Uromodulin) Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Recombinant human UMOD protein, N-His probed with UMOD Polyclonal Antibody, Unconjugated (bs-2189R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.