bs-2162R-BF680 [Conjugated Primary Antibody]
DKK1 Polyclonal Antibody, AbBy Fluor® 680 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DKK1

Immunogen Range: 201-266/266


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 22943

Swiss Prot: O94907

Source: KLH conjugated synthetic peptide derived from human DKK1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Conjugation: AbBy Fluor® 680

Excitation/ Emission: 679nm/702nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Dog

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995.Read more>>
  • Lu, Juan, et al. "Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." Acta Odontologica Scandinavica (2015): 1-7.Read more>>
  • Duan, Hongying, et al. "TET1 inhibits EMT of ovarian cancer cells through activating Wnt/β-catenin signaling inhibitors DKK1 and SFRP2." Gynecologic Oncology (2017).Read more>>
  • Elena E. Pakhomova. et al. Comparative Evaluation of the Clinical Efficacy of PRP-Therapy, Minoxidil, and Their Combination with Immunohistochemical Study of the Dynamics of Cell Proliferation in the Treatment of Men with Androgenetic Alopecia. Int J Mol Sci. 2020 Jan;21(18):6516Read more>>
  • Hai Zhao. et al. Oxidative stress caused by a dysregulated Wnt/-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome. Zygote. 2020 Oct;:1-8Read more>>
VALIDATION IMAGES