bs-21385R [Primary Antibody]
RSPO1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: RSPO1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 284654

Swiss Prot: Q2MKA7

Source: KLH conjugated synthetic peptide derived from human RSPO1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

RSPO1 is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(IHC-P(1:200-400))
IF(ICC)(IF(ICC)(1:50-200))

Predicted Molecular Weight: 27


Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Pig
Rabbit
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jinmiao Tian. et al. QPH-FR: A Novel Quinoa Peptide Enhances Chemosensitivity by Targeting Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5 in Colorectal Cancer. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4°C, DAB staining.