Host: Rabbit
Target Protein: Cyp2-j3
Immunogen Range: 401-502/502
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1573
Swiss Prot: P51589
Source: KLH conjugated synthetic peptide derived from human Cyp2-j3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 55
Cross Reactive Species: Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
- You et al. Ophiopogonin D maintains Ca2+ homeostasis in rat cardiomyocytes in vitro by upregulating CYP2J3/EETs and suppressing ER stress. (2016) Acta.Pharmacol.Sin. 37:368-81Read more>>
- Huang X et al. Ophiopogonin D Reduces Myocardial Ischemia-Reperfusion Injury via Upregulating CYP2J3/EETs in Rats.(2018) Cellular Physiology and Biochemistry.49(4):1646-1658.. Read more>>
- Zhang J et al. EETs/PPARs activation together mediates the preventive effect of naringenin in high glucose-induced cardiomyocyte hypertrophy.(2019)Biomed Pharmacother;Jan;109:1498-1505.Read more>>
- Wang J et al. Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 UpregulationOxid Med Cell Longev.2020 Dec 31;2020:8857906.Read more>>
Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 55kD. Observed band size: 55kD.
Formalin-fixed and paraffin embedded mouse heart labeled with Anti-Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Liver lysates; Lane 2: Brain lysates probed with Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.