bs-2122R [Primary Antibody]
GST tag Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GST tag

Clonality: Polyclonal

Isotype: IgG

Source: Recombinant full length GST tag protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 26


Cross Reactive Species: Tags
Others
(GST fusion protein)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cao, Meng, et al. "The formation of intracellular nanoparticles correlates with cisplatin resistance." Science China Materials (2015): 1-9.Read more>>
  • Qing Li. et al. SAMD9 Promotes Postoperative Recurrence of Esophageal Squamous Cell Carcinoma by Stimulating MYH9-Mediated GSK3/-Catenin Signaling. Advanced Science. 2023 Feb;:2203573Read more>>
VALIDATION IMAGES

Lane 1: GST-Tagged Fusion Protein Overexpression E.coli lysates; Lane 2: GST-Tagged Fusion Protein Overexpression E.coli lysates probed with GST tag Polyclonal Antibody, Unconjugated (bs-2122R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: GST-tagged fusion protein probed with GST tag Polyclonal Antibody, Unconjugated (bs-2122R) at 1:60000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bs-2122R (Anti-GST) at dilution of 1:60,000 incubated at 4℃ overnight.