bs-2096R [Primary Antibody]
kNBC Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: kNBC

Immunogen Range: 1-100/1035


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 54403

Swiss Prot: O88343

Source: KLH conjugated synthetic peptide derived from Mouse electrogenic sodium bicarbonate cotransporter isoform 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

SLC4A4 (Electrogenic sodium bicarbonate cotransporter 1) is an electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. It may regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. SLC4A4 interacts with carbonic anhydrase 2 and carbonic anhydrase 4 which may regulate transporter activity. There are four named isoforms produced by alternative splicing.This gene encodes a sodium bicarbonate cotransporter (NBC) involved in the regulation of bicarbonate secretion and absorption and intracellular pH. Mutations in this gene are associated with proximal renal tubular acidosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 116


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu M et al. Copper promotes sheep pancreatic duct organoid growth by ATOX1-dependent MEK-ERK pathway activation. Am J Physiol Cell Physiol. 2020 Apr 1;318(4):C806-C816. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-SLC4A4 Polyclonal Antibody, Unconjugated(bs-2096R) at 1:400 followed by conjugation to the secondary antibody and DAB staining


Kidney lysates probed with kNBC Polyclonal Antibody, Unconjugated (bs-2096R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.