bs-2090R-A488
[Conjugated Primary Antibody]
P53(wt-p53) Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated
DATASHEET
Host: Rabbit
Target Protein: P53wt-p53
Immunogen Range: 301-393/393
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7157
Swiss Prot: P04637
Source: KLH conjugated synthetic peptide derived from human P53
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Conjugation: ALEXA FLUOR® 488
Excitation/ Emission: 490nm/525nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 53
Cross Reactive Species:
Human
Mouse
Rat
Goat
Predicted Cross Reactive Species:
Dog
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yang et al. Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma. (2014) Exp.Ther.Me. 8:563-566Read more>>
- Yang et al. RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells. (2011) J.Biol.Che. 286:11865-74Read more>>
- Chen et al. STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7. (2015) Cancer.Cell.Int. 15:111Read more>>
- Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.Read more>>
- Yang,et al.GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial-mesenchymal transition through the TGF-β1/Smad2 signalling pathway.(2018) Journal of Cellular and Molecular Medicine. 22:1650-1665.Read more>>
- Colas,et al.Impaired Production and Diurnal Regulation of Vascular RvDn-3 DPA Increase Systemic Inflammation and Cardiovascular Disease.(2018) Circulation Research. 122:855-863.Read more>>
- Gao D et al. Protective effect of astaxanthin against contrast-induced acute kidney injury via SIRT1-p53 pathway in rats.(2018)Int Urol NephrolRead more>>
- Zhang Y et al. Comparative study on seasonal hair follicle cycling by analysis of the transcriptomes from cashmere and milk goats. Genomics. 2019 Feb 16. pii: S0888-7543(18)30576-7. Read more>>
- Zhao Y et al. Walnut Polyphenol Extract Protects against Malathion- and Chlorpyrifos-Induced Immunotoxicity by Modulating TLRx-NOX-ROS. Nutrients. 2020 Feb 27;12(3). pii: E616. Read more>>
- Danlu Yang. et al. -elemene promotes the senescence of glioma cells through regulating YAP-CDK6 signaling. Am J Cancer Res. 2021; 11(2): 370C388Read more>>
- Xingxing Xu. et al. D-galactose induces senescence of glioblastoma cells through YAP-CDK6 pathway. Aging-Us. 2020 Sep 30; 12(18): 18501C18521Read more>>
- Zihao Feng. et al. Bioinformatics and experimental findings reveal the therapeutic actions and targets of pachymic acid against cystitis glandularis. 2021 Apr 24Read more>>
- Zixuan Liu. et al. Iron-bearing nanoparticles trigger human umbilical vein endothelial cells ferroptotic responses by promoting intracellular iron level. Environ Pollut. 2021 Oct;287:117345Read more>>
- Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brainCliver axis in rats. 2021 May 18Read more>>
- Xingxing Xu. et al. YAP prevents premature senescence of astrocytes and cognitive decline of Alzheimer's disease through regulating CDK6 signaling. 2021 Aug 2Read more>>
- Yu, Ting-Ting. et al. Harnessing chlorin e6 loaded by functionalized iron oxide nanoparticles linked with glucose for target photodynamic therapy and improving of the immunogenicity of lung cancer. J Cancer Res Clin. 2022 Jan;:1-13Read more>>
- Ning Han. et al. Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS. Eur J Pharmacol. 2022 Feb;:174797Read more>>
- Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784Read more>>
- Yang, Xiao-Xin. et al. A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin. J NANOBIOTECHNOL. 2022 Dec;20(1):1-19Read more>>
- Liu-Gen Li. et al. Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage. FRONT PHARMACOL. 2022; 13: 949835Read more>>
- Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682Read more>>
- Ting-Ting Yu. et al. Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage. INT IMMUNOPHARMACOL. 2023 Feb;115:109661Read more>>
- Qi-Rui Li. et al. Laser-triggered intelligent drug delivery and anti-cancer photodynamic therapy using platelets as the vehicle. PLATELETS. 2023;34(1):Article: 2166677Read more>>
- Yin Yang. et al. Toosendanin-induced apoptosis in canine mammary tumor CMT-U27 cells is mediated through the mitochondrial apoptotic pathway. VET COMP ONCOL. 2023 FebRead more>>
- Yuan-Jian Hui. et al. Up-regulation of ABCG2 by MYBL2 deletion drives Chlorin e6-mediated photodynamic therapy resistance in colorectal cancer. PHOTODIAGN PHOTODYN. 2023 Apr;:103558Read more>>
- Liu-Gen Li. et al. A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage. ADV HEALTHC MATER. 2023 Aug;:2301561Read more>>
- Qiuxiang Cai. et al. Pasteurella multocida causes liver injury in ducks by mediating inflammatory, apoptotic and autophagic pathways. MICROB PATHOGENESIS. 2023 Sep;:106336Read more>>
- Zi-Yi Yang. et al. Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy. PHOTODIAGN PHOTODYN. 2023 Nov;:103917Read more>>
- Xue Zhang. et al. Homoharringtonine induces apoptosis of mammary carcinoma cells by inhibiting the AKT/mTOR signaling pathway. VET COMP ONCOL. 2023 DecRead more>>
- Hui Yuan-Jian. et al. B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer. SCI REP-UK. 2024 Apr;14(1):1-15Read more>>
- Li Liu-Gen. et al. Dihydroartemisinin-driven selective anti-lung cancer proliferation by binding to EGFR and inhibition of NRAS signaling pathway-induced DNA damage. SCI REP-UK. 2024 May;14(1):1-15Read more>>
VALIDATION IMAGES