bs-2081R [Primary Antibody]
TNF alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNF alpha

Immunogen Range: 181-235/235


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 21926

Swiss Prot: P06804

Source: KLH conjugated synthetic peptide derived from mouse TNF-alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)

Predicted Molecular Weight: 17/26


Cross Reactive Species: Human
Mouse
Rat
Rabbit
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Morishita, Seita, et al. ʺSystemic Simvastatin Rescues Retinal Ganglion Cells from Optic Nerve Injury Possibly through Suppression of Astroglial NF-κB Activation.ʺ PloS one 9.1 (2014): e84387.Read more>>
  • Wang, Shan, et al. "5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts." Ultrasound in Medicine & Biology (2014).Read more>>
  • Sun, Chao, et al. "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." BioMed Research International 2015 (2015).Read more>>
  • Wang, Yi, et al. "Targeted knockout of TNF‑α by injection of lentivirus‑mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." Molecular Medicine Reports.Read more>>
  • Xu, Qiang, et al. "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." BMC Ophthalmology 15.1 (2015): 126.Read more>>
  • Yue, Yuan, et al. "Synergistic inhibitory effects of naproxen in combination with magnolol on TPA-induced skin inflammation in mice." RSC Advances6.44 (2016): 38092-38099.Read more>>
  • Ji, Mingli, et al. "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits."Experimental and Therapeutic Medicine 12.1 (2016): 51-58.Read more>>
  • Shi, Xiaowei, et al. "Panax notoginseng saponins administration modulates pro-/anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." Metabolic Brain Disease (2016): 1-13.Read more>>
  • Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166.Read more>>
  • Dong, Min, et al. "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE−/− mice." Biochemical and Biophysical Research Communications (2017).Read more>>
  • Han, Sheng, et al. "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83.Read more>>
  • Wang, Xinhong, et al. "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." Cytokine (2017).Read more>>
  • Fang et al. Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption. (2013) Int.J.Mol.Sc. 14:10343-54Read more>>
  • Luo et al. Maternal Bisphenol A Diet Induces Anxiety-like Behavior in Female Juvenile with Neuroimmune Activation. (2014) Toxicol.Sc. Read more>>
  • Wang et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin?induced lung inflammation in a murine model of asthma. (2016) Mol.Med.Rep. 13:4289-302Read more>>
  • Bertuglia et al. Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career. (2016) BMC.Vet.Res. 12:246Read more>>
  • Lennikov et al. Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis. (2018) Angiogenesis. Read more>>
  • Li et al. Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation. (2017) Mol.Med.Rep. 16:4455-4462Read more>>
  • Qian et al. FSH aggravates bone loss in ovariectomised rats with experimental periapical periodontitis. (2016) Mol.Med.Rep. 14:2997-3006Read more>>
  • Ding,et al.ZEB2 Attenuates LPS-Induced Inflammation by the NF-κB Pathway in HK-2 Cells.(2018) Inflammation. 41:722-731.Read more>>
  • Kim,et al.Local administration of nuclear factor of activated T cells (NFAT) c1 inhibitor to suppress early resorption and inflammation induced by bone morphogenetic protein-2.(2018) Journal of Biomedical Materials Research. Part A. 106:1299-1310.Read more>>
  • Wang,et al.Cx43 and NMDA receptors changes in UL122 genetically modified mice hippocampus: a mechanism for spatial memory impairment.(2018) International Journal of Clinical and Experimental Pathology. 11:129-1379.Read more>>
  • Luo Y et al. MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Brain Res Bull. 2019 Mar;146:320-326. Read more>>
  • Jena PK et al. Long-term Western diet intake leads to dysregulated bile acid signaling and dermatitis with Th2 and Th17 pathway features in mice. J Dermatol Sci. 2019 Jun 8. pii: S0923-1811(19)30145-8.Read more>>
  • Hou DD et al. Anti-inflammatory effects of quercetin in a mouse model of MC903-induced atopic dermatitis. Int Immunopharmacol. 2019 Jun 7;74:105676.Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Zhang J et al. Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation. Toxicology. 2019 Mar 1;415:37-48.Read more>>
  • Ma WJ et al. Biliary antibiotics irrigation for E. coli-induced chronic proliferative cholangitis and hepatolithiasis: A pathophysiological study in rabbits. Clin Res Hepatol Gastroenterol. 2019 Aug 13. pii: S2210-7401(19)30173-1.Read more>>
  • Gao X et al. Inhibition of LOX-1 prevents inflammation and photoreceptor cell death in retinal degeneration. Int Immunopharmacol. 2020 Mar;80:106190. Read more>>
  • Luo Q et al. Iron Overload Resulting from the Chronic Oral Administration of Ferric Citrate Impairs Intestinal Immune and Barrier in Mice. Biol Trace Elem Res. 2020 May 28.Read more>>
  • Kumar A et al. Nimbolide inhibits tumor growth by restoring hepatic tight junction protein expression and reduced inflammation in an experimental hepatocarcinogenesis. World J Gastroenterol.2020 Dec 7;26(45):7131-7152.Read more>>
  • Mengmeng Yang. et al. Mesenchymal stem cell-conditioned medium improved mitochondrial function and alleviated inflammation and apoptosis in non-alcoholic fatty liver disease by regulating SIRT1. Biochem Bioph Res Co. 2021 Mar;546:74Read more>>
  • Leandra B. Jones. et al. Lipopolysaccharide Administration Alters Extracellular Vesicles in Cell Lines and Mice. 2021 Feb 09Read more>>
  • Xinran Li. et al. Fumonisins B1 exposure triggers intestinal tract injury via activating nuclear xenobiotic receptors and attracting inflammation response. Environ Pollut. 2020 Dec;267:115461Read more>>
  • Chinthalapally V. Rao. et al. GSK3\ARC/Arg3.1 and GSK3\Wnt signaling axes trigger amyloid\ accumulation and neuroinflammation in middle\aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221Read more>>
  • H.Q. Hu. et al. The STING-IRF3 pathway is involved in lipotoxic injury of pancreatic cells in type 2 diabetes. Mol Cell Endocrinol. 2020 Dec;518:11089Read more>>
  • Mehmet Balbaba. et al. Anti-inflammatory effect of cortistatin in rat endotoxin-induced uveitis model. Indian J Ophthalmol. 2020 Sep; 68(9): 1920C1924Read more>>
  • Huo Shengqi. et al. Alleviation of Inflammation and Oxidative Stress in Pressure Overload-Induced Cardiac Remodeling and Heart Failure via IL-6/STAT3 Inhibition by Raloxifene. Oxid Med Cell Longev. 2021;2021:6699054Read more>>
  • Yixin Cui. et al. Melatonin prevents diabetes\associated cognitive dysfunction from microglia\mediated neuroinflammation by activating autophagy via TLR4/Akt/mTOR pathway. Faseb J. 2021 Apr;35(4):e21485Read more>>
  • Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 ApRead more>>
  • Fang Wang. et al. Stratifin promotes renal dysfunction in ischemic and nephrotoxic AKI mouse models via enhancing RIPK3-mediated necroptosis. Acta Pharmacol Sin. 2021 Apr;:1-12Read more>>
  • Zihao Feng. et al. Bioinformatics and experimental findings reveal the therapeutic actions and targets of pachymic acid against cystitis glandularis. 2021 Apr 24Read more>>
  • Canzhang Li. et al. A novel method to establish the rabbit model of knee osteoarthritis: intra-articular injection of SDF-1 induces OA. Bmc Musculoskel Dis. 2021 Dec;22(1):1-1Read more>>
  • Jiayi Li. et al. Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis. Environ Pollut. 2021 Oct;286:117319Read more>>
  • Gaolong Zhong. et al. Arsenic exposure induces intestinal barrier damage and consequent activation of gut-liver axis leading to inflammation and pyroptosis of liver in ducks. Sci Total Environ. 2021 Sep;788:14778Read more>>
  • in Feng. et al. Li-ESWT treatment reduces inflammation, oxidative stress, and pain via the PI3K/AKT/FOXO1 pathway in autoimmune prostatitis rat models. 2021 May 07Read more>>
  • Randa S. Eshaq. et al. The role of tumor necrosis factor- and interferon- in the hyperglycemia-induced ubiquitination and loss of platelet endothelial cell adhesion molecule-1 in rat retinal endothelial cells. 2021 May 29Read more>>
  • Abdulkareem Aljumaily, Sara Asaad. et al. Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats. 2021 Jul 28Read more>>
  • Emin Kaymak. et al. Thymoquinone has a neuroprotective effect against inflammation, oxidative stress, and endoplasmic reticulum stress in the brain cortex, medulla, and hippocampus due to doxorubicin. 2021 Aug 15Read more>>
  • Sheng-Han Lee. et al. Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimers disease mouse model. Plos One. 2021 Aug;16(8):e0254587Read more>>
VALIDATION IMAGES

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated (bs-2081R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Lane 1: Human A431 cell lysates; Lane 2: Human Hela cell lysates probed with TNF alpha Polyclonal Antibody, Unconjugated (bs-2081R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.