bs-20763R [Primary Antibody]
PARP1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PARP1

Immunogen Range: 581-650/1014


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6352

Swiss Prot: P09874

Source: KLH conjugated synthetic peptide derived from human PARP1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Flow-Cyt(Flow-Cyt=1ug/Test)

Predicted Molecular Weight: 112


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xin Shan. et al. Proteomic analysis of healthy and atretic porcine follicular granulosa cells. J Proteomics. 2021 Feb;232:104027Read more>>
  • Cuicui Zhuang. et al. Escherichia coli infection mediates pyroptosis via activating p53-p21 pathway-regulated apoptosis and cell cycle arrest in bovine mammary epithelial cells. MICROB PATHOGENESIS. 2023 Sep;:106338Read more>>
VALIDATION IMAGES

Mouse Testis lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-20763R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Human K562 cell lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human Hela cell lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-20763R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-20763R) at 1:200 overnight at 4°C, DAB staining.